Detailed information of TA system TA183698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 2550515..2551110
• Replicon: chromosome
• Accession: NZ_CP065966
• Organism: Pseudomonas aeruginosa strain FDAARGOS_1041

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: I6H98_RS12075
• Protein ID: WP_003117425.1
• Coordinates: 2550832..2551110 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6H98_RS12070
• Protein ID: WP_003099268.1
• Coordinates: 2550515..2550820 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H98_RS12040	2545968..2546258	-	291	WP_023083237.1	DUF5447 family protein	-
I6H98_RS12045	2546470..2546742	-	273	WP_003115921.1	hypothetical protein	-
I6H98_RS12050	2546852..2547118	+	267	WP_016852153.1	hypothetical protein	-
I6H98_RS12055	2547241..2548086	+	846	WP_196645791.1	helix-turn-helix domain-containing protein	-
I6H98_RS12060	2548061..2549599	+	1539	WP_023082696.1	GNAT family N-acetyltransferase	-
I6H98_RS12065	2549611..2550138	-	528	WP_071535723.1	AAA family ATPase	-
I6H98_RS12070	2550515..2550820	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
I6H98_RS12075	2550832..2551110	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6H98_RS12080	2551439..2553667	+	2229	WP_003099265.1	TonB-dependent receptor	-
I6H98_RS12085	2553737..2554384	-	648	WP_003095021.1	carbonate dehydratase	-
I6H98_RS12090	2554446..2555684	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T183698 WP_003117425.1 NZ_CP065966:c2551110-2550832 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T183698 NZ_CP087162:c8821-8519 [Pseudomonas sp. CIP-10]
ATGAAGACAATTAAACAAACCGCCACGTACAGGAACTGGGAACGCAAGCTGCGAGACAAGCAGGCCAAAGCCATCATCGC
TGCGCGAGTCTTCCGCGTTGCGCATGGTCTGCTTGGAGACGTTCAACCAGTGGGCCAAGGGATCAGCGAACTGCGTATCC
ACCACGGCCCCGGCTACCGCGTGTATTTCCAGCAGCGCGGTAACCAGCTGGTCCTGTTGCTGTGTGGTGGGGACAAGAGC
AGCCAAGCGCGGGATATCGAAACCGCCAAGTCCTTGGCCAGCCAGTGGAGTGACGATGAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT183698 WP_003099268.1 NZ_CP065966:c2550820-2550515 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT183698 NZ_CP087162:c8522-8223 [Pseudomonas sp. CIP-10]
ATGAAAGAACAATTTTACGACTACGATCCCGCTGATGCGCTGACCGATGCTGAGTCCATCGCAGTATTCATGGAGGACGC
ATTCGAAACAGGCGATGCGGCCTATATCGCAAAATGCCTGGGTGTCGTGGCCCGCGCCAAAGGGATGACCGCCCTTGCCA
ATGAATCTGGCCTTTCCCGCGAACACCTTTACCGGTCGTTCTCGGACAAGGGCAATCCGACCTTGAAAACCCTGCTGGCC
GTGATGCGTGCCGTTGGCCTGGACCTGACAACACGCAAGCACGTTGAACCGGCACACTAA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5