Detailed information of TA system TA183657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 3107482..3108077
• Replicon: chromosome
• Accession: NZ_CP065947
• Organism: Pseudomonas aeruginosa strain PAS6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: JBL36_RS14520
• Protein ID: WP_003117425.1
• Coordinates: 3107482..3107760 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JBL36_RS14525
• Protein ID: WP_003099268.1
• Coordinates: 3107772..3108077 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JBL36_RS14505 (JBL36_14505)	3102908..3104146	+	1239	WP_003113524.1	dipeptidase	-
JBL36_RS14510 (JBL36_14510)	3104208..3104855	+	648	WP_003095021.1	carbonate dehydratase	-
JBL36_RS14515 (JBL36_14515)	3104925..3107153	-	2229	WP_003099265.1	TonB-dependent receptor	-
JBL36_RS31645	3107301..3107429	+	129	Protein_2868	integrase	-
JBL36_RS14520 (JBL36_14520)	3107482..3107760	+	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JBL36_RS14525 (JBL36_14525)	3107772..3108077	+	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
JBL36_RS14535 (JBL36_14535)	3108474..3109574	-	1101	WP_003099270.1	redox-regulated ATPase YchF	-
JBL36_RS14540 (JBL36_14540)	3109615..3110199	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
JBL36_RS14545 (JBL36_14545)	3110241..3110855	-	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
JBL36_RS14550 (JBL36_14550)	3110972..3111913	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
JBL36_RS14560 (JBL36_14560)	3112080..3112928	-	849	WP_003117426.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T183657 WP_003117425.1 NZ_CP065947:3107482-3107760 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T183657 NZ_CP087146:349765-350112 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT183657 WP_003099268.1 NZ_CP065947:3107772-3108077 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT183657 NZ_CP087146:350112-350411 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5