Detailed information of TA system TA183653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 4243859..4244361
• Replicon: chromosome
• Accession: NZ_CP065946
• Organism: Pectobacterium parmentieri strain WC19161

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A8B3FFY4
• Locus tag: JBL47_RS19355
• Protein ID: WP_025919527.1
• Coordinates: 4244107..4244361 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: JBL47_RS19350
• Protein ID: WP_025919528.1
• Coordinates: 4243859..4244110 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JBL47_RS19335	4240281..4241240	+	960	WP_012822281.1	ABC transporter permease	-
JBL47_RS19340	4241233..4242099	+	867	WP_012822280.1	ABC transporter permease	-
JBL47_RS19345	4242096..4243784	+	1689	WP_025919529.1	ABC transporter ATP-binding protein	-
JBL47_RS19350	4243859..4244110	+	252	WP_025919528.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
JBL47_RS19355	4244107..4244361	+	255	WP_025919527.1	Txe/YoeB family addiction module toxin	Toxin
JBL47_RS19360	4244422..4245273	-	852	WP_198481846.1	helix-turn-helix domain-containing protein	-
JBL47_RS19365	4245298..4246137	-	840	WP_198481847.1	HTH-type transcriptional activator RhaS	-
JBL47_RS19370	4246506..4247996	+	1491	WP_012822275.1	rhamnulokinase	-
JBL47_RS19375	4247993..4249255	+	1263	WP_012822274.1	L-rhamnose isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10135.66 Da
• Isoelectric Point: 8.0367

>T183653 WP_025919527.1 NZ_CP065946:4244107-4244361 [Pectobacterium parmentieri]
VKPIWSEAAWEDYLYWQDIDNRIVKKINELIKEIRRTPFEGKGKPEPLKHNLAGFWSRRITEEHRLVYAITDDAMMIAAC
RYHY

Nucleotide

• Length: 255 bp

>T183653 NZ_CP087143:c124607-124164 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9279.58 Da
• Isoelectric Point: 4.7854

>AT183653 WP_025919528.1 NZ_CP065946:4243859-4244110 [Pectobacterium parmentieri]
MRTISYSEARQNLSATMMKTVEDRAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANAKRLMNAIESLKAGNGEERD
IIG

Nucleotide

• Length: 252 bp

>AT183653 NZ_CP087143:c124834-124604 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A8B3FFY4