Detailed information of TA system TA183645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1239257..1239951
• Replicon: chromosome
• Accession: NZ_CP065946
• Organism: Pectobacterium parmentieri strain WC19161

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: JBL47_RS05690
• Protein ID: WP_198482498.1
• Coordinates: 1239553..1239951 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: G7LPQ3
• Locus tag: JBL47_RS05685
• Protein ID: WP_009111694.1
• Coordinates: 1239257..1239550 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JBL47_RS05660	1234339..1234517	+	179	Protein_1107	transposase	-
JBL47_RS05665	1234689..1235225	+	537	WP_198482235.1	SLATT domain-containing protein	-
JBL47_RS05670	1235222..1236595	+	1374	WP_198482236.1	RNA-directed DNA polymerase	-
JBL47_RS05675	1236782..1237294	-	513	WP_198482237.1	hypothetical protein	-
JBL47_RS05680	1237418..1238935	-	1518	WP_198482238.1	RNA-directed DNA polymerase	-
JBL47_RS05685	1239257..1239550	+	294	WP_009111694.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JBL47_RS05690	1239553..1239951	+	399	WP_198482498.1	type II toxin-antitoxin system YafO family toxin	Toxin
JBL47_RS05695	1240266..1240955	+	690	Protein_1114	integrase arm-type DNA-binding domain-containing protein	-
JBL47_RS05700	1241036..1242129	+	1094	Protein_1115	IS3 family transposase	-
JBL47_RS05705	1242275..1242499	+	225	WP_198482239.1	tail fiber assembly protein	-
JBL47_RS05710	1242620..1242724	-	105	Protein_1117	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
JBL47_RS05715	1242724..1243017	+	294	Protein_1118	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1191003..1244961	53958
	-	flank	IS/Tn	-	-	1234339..1234503	164

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15224.53 Da
• Isoelectric Point: 7.5155

>T183645 WP_198482498.1 NZ_CP065946:1239553-1239951 [Pectobacterium parmentieri]
IRIFKSALIRQQLSQQELDALAADFLSYKKDGVLPDTFGRDAPYDDDRTYPLVKEEQVAHIHLADADAPFPKFLRQFKRT
SDQAHLVYCQGAMDPDAYLLIIILKPEAHKMARNNNHMHKIGMMAEAFRMKH

Nucleotide

• Length: 399 bp

>T183645 NZ_CP087142:4198798-4199016 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10993.46 Da
• Isoelectric Point: 4.9167

>AT183645 WP_009111694.1 NZ_CP065946:1239257-1239550 [Pectobacterium parmentieri]
MERILAEKSINITELRKNPAKYFIDEPVAVLSNNRPAGYMVSAKVFEELIDLLEEKQGRVHTAARFRPTAERLSDIADSG
QKLLQNASDKDLTEFTE

Nucleotide

• Length: 294 bp

>AT183645 NZ_CP087142:4198397-4198771 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A2U1UKC7