Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1258917..1259644 Replicon chromosome
Accession NZ_CP065939
Organism Enterobacter hormaechei strain MSB1_8J

Toxin (Protein)


Gene name higB Uniprot ID A0A8B3V067
Locus tag JDC97_RS05980 Protein ID WP_022650547.1
Coordinates 1259333..1259644 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B3V464
Locus tag JDC97_RS05975 Protein ID WP_022650546.1
Coordinates 1258917..1259336 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JDC97_RS05960 1255989..1257110 - 1122 WP_003858868.1 glutamate--cysteine ligase -
JDC97_RS05965 1257216..1258106 + 891 WP_022650544.1 oxidoreductase -
JDC97_RS05970 1258159..1258593 - 435 WP_022650545.1 hypothetical protein -
JDC97_RS05975 1258917..1259336 - 420 WP_022650546.1 helix-turn-helix domain-containing protein Antitoxin
JDC97_RS05980 1259333..1259644 - 312 WP_022650547.1 type II toxin-antitoxin system HigB family toxin Toxin
JDC97_RS05985 1260337..1260756 + 420 WP_032645895.1 hypothetical protein -
JDC97_RS05990 1260869..1261324 - 456 WP_022650549.1 hypothetical protein -
JDC97_RS05995 1261607..1262488 - 882 WP_022650550.1 LysR family transcriptional regulator -
JDC97_RS06000 1262594..1263373 + 780 WP_003858848.1 acetolactate decarboxylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12554.34 Da        Isoelectric Point: 9.9435

>T183630 WP_022650547.1 NZ_CP065939:c1259644-1259333 [Enterobacter hormaechei]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Download         Length: 312 bp

>T183630 NZ_CP087136:c3692617-3692510 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15447.51 Da        Isoelectric Point: 4.6051

>AT183630 WP_022650546.1 NZ_CP065939:c1259336-1258917 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp

>AT183630 NZ_CP087136:3692666-3692732 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3V067

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3V464

References