Detailed information of TA system TA183572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1239634..1240256
• Replicon: chromosome
• Accession: NZ_CP065867
• Organism: Pseudomonas aeruginosa strain TJ2014-049

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I9X29_RS05815
• Protein ID: WP_023910260.1
• Coordinates: 1239634..1239948 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I9X29_RS05820
• Protein ID: WP_023910258.1
• Coordinates: 1239951..1240256 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9X29_RS05790	1235294..1236295	+	1002	WP_079753611.1	site-specific integrase	-
I9X29_RS05795	1236364..1236675	+	312	WP_031631670.1	outer membrane protein assembly factor BamE	-
I9X29_RS05800	1236807..1237457	+	651	WP_058172679.1	BRCT domain-containing protein	-
I9X29_RS05805	1237642..1238028	+	387	WP_198341193.1	hypothetical protein	-
I9X29_RS05810	1238315..1239058	+	744	WP_058172667.1	DUF3800 domain-containing protein	-
I9X29_RS05815	1239634..1239948	+	315	WP_023910260.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I9X29_RS05820	1239951..1240256	+	306	WP_023910258.1	helix-turn-helix domain-containing protein	Antitoxin
I9X29_RS05825	1240317..1240616	-	300	WP_058172668.1	hypothetical protein	-
I9X29_RS05830	1240717..1240929	-	213	WP_121333665.1	hypothetical protein	-
I9X29_RS05835	1240926..1242806	-	1881	WP_107113824.1	DNA cytosine methyltransferase	-
I9X29_RS05840	1242803..1243282	-	480	WP_058172361.1	hypothetical protein	-
I9X29_RS05845	1243292..1243531	-	240	WP_015649068.1	hypothetical protein	-
I9X29_RS05850	1243528..1244154	-	627	WP_019396881.1	hypothetical protein	-
I9X29_RS05855	1244205..1244522	-	318	WP_019396880.1	pyocin activator PrtN family protein	-
I9X29_RS05860	1244519..1244749	-	231	WP_019726488.1	hypothetical protein	-
I9X29_RS05865	1244900..1245193	-	294	WP_019396879.1	phage antirepressor KilAC domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1235339..1278090	42751

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-72)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11776.54 Da
• Isoelectric Point: 9.4385

>T183572 WP_023910260.1 NZ_CP065867:1239634-1239948 [Pseudomonas aeruginosa]
MIFIETPVFTKRILALVDDETYRKLQEDLTLHPDAGVVIEGTGGVRKIRIAANGHGKRGGARVIYYHFTSASQIAFLLAY
DKASQEDLTADQKKMLRQIVENWR

Nucleotide

• Length: 315 bp

>T183572 NZ_CP087114:1341098-1341412 [Bartonella machadoae]
ATGATACATAAAAGAACGAATGAAGGTGATTTTGTGATTGAATCTTTTGCGGATAAGCGGTGTAAAGATCTTTTAGAAGG
GAACCCGCCCAGAGGCTTTCCTTCAACTCTAGTGCGTATAGCCCAAAGAAAATTATTTATGCTTGATAAGGCAGTTGATC
TCAAGGATTTGCGCAGTCCTCCGGGAAATCGTCTAGAGGCATTGAAAGGAGAACGTAAAGGTCAATATTCTATTCGTATT
AATGACCAGTTTCGTATTTGTTTTGAATGGCGTTCTAATGGCGCCTATGAAGTTGAAATTGTAGATTATCATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11316.84 Da
• Isoelectric Point: 7.2077

>AT183572 WP_023910258.1 NZ_CP065867:1239951-1240256 [Pseudomonas aeruginosa]
MDKELFAELVESVTQMDEIVRGERQPSREFHVDAVKVKEIRRATGLSQAKFARKIDVAVGTLRNWEQGRRDPEGPARALL
RAIDNDPVHVLAALNGGPRES

Nucleotide

• Length: 306 bp

>AT183572 NZ_CP087114:1341429-1341725 [Bartonella machadoae]
ATGAAAAATTATATAGCAATACACCCCGGAGAAATTTTACGAGAAGAATATTTAAAAGAATATGCTCTTTCTGCTTATGC
TCTTGCAAAAGCATTGAATGTTCCACGCACGAGGATAGAACGGATTGTTGCTGAAAACAGTCCAGTCACTCCCGATACAG
CACTAAGATTAGCCTATTTTTTTGATACCACTGCTGAATTTTGGCTTAACATGCAAGCTGCTTACGACGTTAGTATATTA
CAAACTGAAAAAGCAGATGAATTTGCTAAAATCAATAAATTTGAATGTAGGGTTTAA