Detailed information of TA system TA183570

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5425067..5425662
• Replicon: chromosome
• Accession: NZ_CP065866
• Organism: Pseudomonas aeruginosa strain TJ2019-017

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: I9X27_RS25130
• Protein ID: WP_003113526.1
• Coordinates: 5425384..5425662 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I9X27_RS25125
• Protein ID: WP_003113527.1
• Coordinates: 5425067..5425372 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9X27_RS25090	5420206..5421054	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
I9X27_RS25100	5421221..5422162	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
I9X27_RS25105	5422279..5422893	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
I9X27_RS25110	5422935..5423519	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
I9X27_RS25115	5423560..5424660	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
I9X27_RS25125	5425067..5425372	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
I9X27_RS25130	5425384..5425662	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I9X27_RS25135	5425991..5428219	+	2229	WP_023086667.1	TonB-dependent receptor	-
I9X27_RS25140	5428289..5428936	-	648	WP_003095021.1	carbonate dehydratase	-
I9X27_RS25145	5428998..5430236	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T183570 WP_003113526.1 NZ_CP065866:c5425662-5425384 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T183570 NZ_CP087114:c1242844-1242563 [Bartonella machadoae]
ATGAAATTGAAAACCTTTAACTTTGAAAAACACCTCACAACACCAGAATCTCAAGAAGTCTTTTTAAACGAAGCCTTCAA
AACTGGTGATGCTGCGCATATCGCAGATGCTCTTGGTATCGTTGCCCGCGCTCAAAATATGAGTGCATTAGCAAAGGAAA
CAAACCGTGAACGCAGTGGTTTATATCGCTCCCTTAGCAAAACGGGTGACCCTAGACTTTCTACTTTAGTTGCTGTGCTC
TCTGCCCTTAATTTGCAATTAAGCGTACAATCTACTCCATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT183570 WP_003113527.1 NZ_CP065866:c5425372-5425067 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT183570 NZ_CP087114:c1243136-1242825 [Bartonella machadoae]
ATGGTAGCGATAATAAAAACAACAGATGAATTTGATGAATGGTTAGAAAAGCTTAAAGACAGAAAAGCTGCCGATATAAT
TCTTGCAAGACTTCATAAAATTCGTTCAGGGCTTTTAGGTGATGTTAAATTTTTTCATGGTATAGGTGAATTACGTATCC
ACTATGGCGCGGGTTACAGGATTTATTTCACTCAAAAAGGTAATAAAATCATTATATTGCTTTGTGGTGGTGACAAATCC
ACACAATCCAAAGATATTCAAAAAGCCTTATCTCTCTTGGAGGACCTTAAAAATGAAATTGAAAACCTTTAA

Structures

Toxin

• AlphaFold DB: V6ALY3