Detailed information of TA system TA183564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 6070112..6070707
• Replicon: chromosome
• Accession: NZ_CP065865
• Organism: Pseudomonas aeruginosa strain TJ2019-022

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: I9X26_RS28930
• Protein ID: WP_003117425.1
• Coordinates: 6070429..6070707 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I9X26_RS28925
• Protein ID: WP_003113527.1
• Coordinates: 6070112..6070417 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9X26_RS28890	6065251..6066099	+	849	WP_009878189.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
I9X26_RS28900	6066266..6067207	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
I9X26_RS28905	6067324..6067938	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
I9X26_RS28910	6067980..6068564	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
I9X26_RS28915	6068605..6069705	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
I9X26_RS28925	6070112..6070417	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
I9X26_RS28930	6070429..6070707	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I9X26_RS28935	6071036..6073264	+	2229	WP_004352671.1	TonB-dependent receptor	-
I9X26_RS28940	6073334..6073981	-	648	WP_003095021.1	carbonate dehydratase	-
I9X26_RS28945	6074043..6075281	-	1239	WP_003161380.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T183564 WP_003117425.1 NZ_CP065865:c6070707-6070429 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T183564 NZ_CP087111:c4024631-4024323 [Pseudomonas palleroniana]
ATGAACACAATCATCACTACCCATGAATTTGATAACTGGCTGAAAGGTCTGAAAGACGTACAGGCACGTCTACGGATCGT
TTCTCGGATCTTGAGTGCAGAGGCGGGTAATTTTGGTGACTGTGAGCCGGTGGGCGAAGGCATCAGCGAAATGCGGATCT
TCGTCGGGCCGGGATACCGTGTGTACTACACGCGCCGTGGCGAGGTCGTTTACCTGCTACTGTGTGGCGGCAATAAGTCA
TCACAGAAACGAGACATCAAGCTTGCCAAATCCATCCTCGATGAGATCGAGCAAGGAGACGTAAAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT183564 WP_003113527.1 NZ_CP065865:c6070417-6070112 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT183564 NZ_CP087111:c4024326-4024045 [Pseudomonas palleroniana]
ATGAGAGAACTCAAAGCATTTGATGCATCCCAGTACCTCACCAACGAGGACGAGATTGCTCAATTCCTGACCATTTCTTT
GGAAGACCCAAACCCTGACGTGTTCCTTCGCGCTCTGCAGGAAGTAGCCAAGGCTCGTGGCATGAGCCAGCTTGCCAAGG
ATGCTGGTATCAATCGTGAGAGCCTGTACAAAGCGTTGGCTCCAGGTGCAAAGCCCCGGTATGACACAGTGTTGAAGCTT
GTGAATGCATTAGGCGTGAAGCTGACGGCACAGCACGTTTAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5