Detailed information of TA system TA183562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5342551..5343232
• Replicon: chromosome
• Accession: NZ_CP065865
• Organism: Pseudomonas aeruginosa strain TJ2019-022

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6AKP0
• Locus tag: I9X26_RS25435
• Protein ID: WP_003111825.1
• Coordinates: 5342867..5343232 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I9X26_RS25430
• Protein ID: WP_003145733.1
• Coordinates: 5342551..5342874 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I9X26_RS25405	5338465..5339103	+	639	WP_003119439.1	hypothetical protein	-
I9X26_RS25410	5339344..5339679	+	336	WP_003140884.1	TM2 domain-containing protein	-
I9X26_RS25415	5339854..5340372	+	519	WP_043156339.1	PAAR domain-containing protein	-
I9X26_RS25420	5340369..5341070	+	702	WP_031630293.1	VRR-NUC domain-containing protein	-
I9X26_RS25425	5341087..5342175	+	1089	WP_033936233.1	DUF3396 domain-containing protein	-
I9X26_RS25430	5342551..5342874	-	324	WP_003145733.1	XRE family transcriptional regulator	Antitoxin
I9X26_RS25435	5342867..5343232	-	366	WP_003111825.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I9X26_RS25440	5343496..5343735	-	240	WP_044060930.1	hypothetical protein	-
I9X26_RS25445	5343942..5344214	+	273	WP_023090041.1	hypothetical protein	-
I9X26_RS25450	5344245..5344670	-	426	WP_003114206.1	ring-cleaving dioxygenase	-
I9X26_RS25455	5344771..5345655	+	885	WP_043156336.1	LysR family transcriptional regulator	-
I9X26_RS25460	5345628..5346581	-	954	WP_003085661.1	LysR family transcriptional regulator	-
I9X26_RS25465	5346802..5347236	+	435	WP_003116494.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5308155..5345655	37500

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13880.24 Da
• Isoelectric Point: 4.8219

>T183562 WP_003111825.1 NZ_CP065865:c5343232-5342867 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T183562 NZ_CP087111:c3113807-3113493 [Pseudomonas palleroniana]
ATGATCCGTTTCAAGAGATCCGCCGAGTTTCAGGAGTGGTTTCATTCGTTGCGCGATAAACCCGCCCGTGGACGAGTACT
GACAAGATTAGATAACGCAACGATGGGCAACTTCGGAGACTGCGAAACCGTTGGAAATGGCGTATCGGAAATGCGCATCC
ACTATGGCCCGGGATACCGGGTTTACTTCATGCGCATAGGTGAGGTGGTTTACCTGCTGTTGATAGGAGGCGATAAATCA
ACGCAACAGCGCGACATTCAGCGTGCGAAACAAATTGCAGATGAGTTTAGAAAAAAAGGAGTGGTCCATGACTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11896.73 Da
• Isoelectric Point: 6.4751

>AT183562 WP_003145733.1 NZ_CP065865:c5342874-5342551 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLHVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT183562 NZ_CP087111:c3113500-3113213 [Pseudomonas palleroniana]
ATGACTGAATTTAAAGATTTCGACATAGCCGAATACCTCAAGACCCCCGAGGACATGGCCGAGTATCTTGAAGCCTCTTT
CGAGGAGGATTCAGGTGACGGATTGCTGATTCGTTCAGCCCTTAATCATATCGCTCGCGCCCAAGGCATGACCCAAATCG
CCCGCGACGCCGGCCTCGGACGCGAAAGCTTGTACAAAGCCCTTTCCAGCACTGGCAATCCCGAATTCGCGACTATCATG
AAGGTCATGAAAGCGCTGGGGCTGAAGCTCCACGCCAGCGCTCGCTGA

Structures

Toxin

• AlphaFold DB: V6AKP0