Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 2484533..2485050 Replicon chromosome
Accession NZ_CP065857
Organism Staphylococcus aureus strain CC1153-MRSA

Toxin (Protein)


Gene name yoeB Uniprot ID T1YC79
Locus tag I1A60_RS12325 Protein ID WP_000113262.1
Coordinates 2484533..2484799 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID T1YB60
Locus tag I1A60_RS12330 Protein ID WP_000584499.1
Coordinates 2484799..2485050 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I1A60_RS12295 2479882..2480662 + 781 Protein_2375 transposase -
I1A60_RS12300 2480728..2481552 - 825 WP_064136627.1 formate/nitrite transporter family protein -
I1A60_RS12305 2481769..2481951 - 183 WP_000230294.1 hypothetical protein -
I1A60_RS12310 2482035..2482502 - 468 WP_072468592.1 SRPBCC domain-containing protein -
I1A60_RS12315 2482688..2484238 - 1551 WP_000727763.1 zinc ABC transporter substrate-binding lipoprotein AdcA -
I1A60_RS12320 2484427..2484498 - 72 Protein_2380 hypothetical protein -
I1A60_RS12325 2484533..2484799 - 267 WP_000113262.1 Txe/YoeB family addiction module toxin Toxin
I1A60_RS12330 2484799..2485050 - 252 WP_000584499.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
I1A60_RS12335 2485215..2485316 - 102 Protein_2383 hypothetical protein -
I1A60_RS12340 2485323..2485922 - 600 WP_072468591.1 DsbA family protein -
I1A60_RS12345 2485941..2486303 - 363 WP_000819843.1 DUF4467 domain-containing protein -
I1A60_RS12350 2486558..2487808 + 1251 WP_044291015.1 FemA/FemB family glycyltransferase FmhA -
I1A60_RS12355 2487905..2488636 - 732 WP_000615461.1 amino acid ABC transporter ATP-binding protein -
I1A60_RS12360 2488633..2489352 - 720 WP_000479576.1 amino acid ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2480239..2480556 317


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-74)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10437.95 Da        Isoelectric Point: 9.9143

>T183550 WP_000113262.1 NZ_CP065857:c2484799-2484533 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Download         Length: 267 bp

>T183550 NZ_CP087110:2476049-2476156 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9433.60 Da        Isoelectric Point: 4.3407

>AT183550 WP_000584499.1 NZ_CP065857:c2485050-2484799 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Download         Length: 252 bp

>AT183550 NZ_CP087110:c2475999-2475936 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L8G
PDB 6L8F
PDB 7CUA
PDB 6L8E

Antitoxin

Source ID Structure
PDB 6L8G
PDB 6L8F
PDB 7BWF
PDB 6L8E
AlphaFold DB T1YB60

References