Detailed information of TA system TA183491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3850936..3851533
• Replicon: chromosome
• Accession: NZ_CP065838
• Organism: Klebsiella quasipneumoniae strain KqPF26

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3T0QUS6
• Locus tag: I8N75_RS18790
• Protein ID: WP_017899012.1
• Coordinates: 3851216..3851533 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I8N75_RS18785
• Protein ID: WP_094309536.1
• Coordinates: 3850936..3851223 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I8N75_RS18755	3847023..3847271	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
I8N75_RS18760	3847289..3847630	-	342	WP_004202492.1	RamA family antibiotic efflux transcriptional regulator	-
I8N75_RS18765	3847661..3848776	-	1116	WP_025999133.1	MBL fold metallo-hydrolase	-
I8N75_RS18770	3848956..3849537	+	582	WP_017899014.1	TetR/AcrR family transcriptional regulator	-
I8N75_RS18775	3849537..3849905	+	369	WP_004202487.1	MmcQ/YjbR family DNA-binding protein	-
I8N75_RS18780	3850025..3850678	+	654	WP_198974676.1	oxygen-insensitive NAD(P)H nitroreductase	-
I8N75_RS18785	3850936..3851223	-	288	WP_094309536.1	helix-turn-helix transcriptional regulator	Antitoxin
I8N75_RS18790	3851216..3851533	-	318	WP_017899012.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I8N75_RS18795	3851718..3852761	-	1044	WP_094309535.1	DUF2157 domain-containing protein	-
I8N75_RS18800	3853294..3854163	-	870	WP_004202474.1	helix-turn-helix transcriptional regulator	-
I8N75_RS18805	3854272..3855699	+	1428	WP_064150543.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-87)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12185.52 Da
• Isoelectric Point: 11.2767

>T183491 WP_017899012.1 NZ_CP065838:c3851533-3851216 [Klebsiella quasipneumoniae]
MFRMVVHVDVKKELQALPAIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQNKTLFLLRVF
VKKTQKTPLSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T183491 NZ_CP087061:c25332-24910 [Pseudosulfitobacter pseudonitzschiae]
ATGATCATCCTCGATACGAATGTCATCTGCGAGCTGTTGCGCCCGACCCCGGAGCCCAGGATCGAGCAATGGCTGTCCGC
TCAAGATGGTCTAAACGTCTACCTGACCTCGATATCGGAGGCGGAGCTGCGCTATGGCCTCGCGAACATGGGGAACGGCA
AGCGCCGCGCTGCGCTGATTGACGCCGTAGACCGCATCCTGCGCGAGGACCTCGCCGGGCGCATTCTGCCCTTCGACAGC
GACGCCGCGCAGGCATACGCCTCTATCGCCGCCACTCGTCGCGACGCCGGGCGACCGATTGCCCAGGCCGATTGCCAGAT
CGCATCCATTGCCCACGCCTGTGGCGCCACCGTCGCCACACGCAATACGCCCGACTTCGAGGGTTGCGAGATTGACCTGA
TCAACCCCTGGACAACCGAATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10810.49 Da
• Isoelectric Point: 10.2213

>AT183491 WP_094309536.1 NZ_CP065838:c3851223-3850936 [Klebsiella quasipneumoniae]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQHRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRTSLDTLAR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT183491 NZ_CP087061:c25584-25336 [Pseudosulfitobacter pseudonitzschiae]
ATGGCAAGCATTACGATCCGGAACCTTGACGATGACGTGAAGCGGCGCCTTCGCATCCGAGCGGCCGAGCATGGCCGTTC
GATGGAAGAGGAGGCGCGAGAGATCCTGCGCCAAGTCGTTGGCGACGCGAAGCCCAACCATGATCTTGCCGCGGCGATCC
GTGCGCGGGTTGGACCCTTGGGTGGCGTTGAACTTGACCTTCCGCAGCGTGAAGCCATGCATGAGCCGCCCACATTCGAC
CGAGCCTAA

Structures

Toxin

• AlphaFold DB: A0A3T0QUS6