Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 337581..338167 Replicon chromosome
Accession NZ_CP065833
Organism Klebsiella pneumoniae strain KpC5

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag I8N78_RS01580 Protein ID WP_002920800.1
Coordinates 337799..338167 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag I8N78_RS01575 Protein ID WP_004174006.1
Coordinates 337581..337802 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8N78_RS01555 333738..334664 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
I8N78_RS01560 334661..335938 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
I8N78_RS01565 335935..336702 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
I8N78_RS01570 336704..337417 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
I8N78_RS01575 337581..337802 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
I8N78_RS01580 337799..338167 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
I8N78_RS01585 338440..339756 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
I8N78_RS01590 339863..340750 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
I8N78_RS01595 340747..341592 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
I8N78_RS01600 341594..342664 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 334661..343401 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T183457 WP_002920800.1 NZ_CP065833:337799-338167 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T183457 NZ_CP087025:3315862-3316125 [Pseudosulfitobacter pseudonitzschiae]
ATGAAGTTCGAGTATGACCCTGACAAGAGTGCCGGGAACAAGGCCAAGCACGGCATCGACTTCGATGAAGCGCAAGCGCT
TTGGGAAGACCCGTGGCTGTTGGAGGCTCCCGCTCGAACAGATGACGAGCCGCGCTTTTTTGCAATAGGCATGATCAAGG
AACGGCATTGGTCAGCAGTTTGCACCTATCGCGGTGATATTATTCGGATCATCTCGGTGCGCCGCGCCAGAAAACAGGAG
ATTGAGCACTATGAAAGCACGTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT183457 WP_004174006.1 NZ_CP065833:337581-337802 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT183457 NZ_CP087025:3316112-3316327 [Pseudosulfitobacter pseudonitzschiae]
ATGAAAGCACGTGAGTTCGATCAAAAATTTGACACAGGTGAGAACGTCAGCGATCTTGTGGACTGGTCCAAAGCTAGGCG
GACGAATACCCAGACGAAGCGAGTCAATGTGGATTTTCCGTCGTGGGTCGTTTCTGGTTTGGATCAGAAGGCAAAGCAGC
TCGGCGTTACTCGTCAGTCGCTCATCAAACTCTGGATCGCCGAGCGGCTCCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References