Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3999860..4000457 Replicon chromosome
Accession NZ_CP065831
Organism Klebsiella pneumoniae strain KpC11

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag I8N76_RS19855 Protein ID WP_004142563.1
Coordinates 4000140..4000457 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag I8N76_RS19850 Protein ID WP_004142561.1
Coordinates 3999860..4000147 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8N76_RS19820 3995940..3996188 + 249 WP_002893037.1 DUF1158 domain-containing protein -
I8N76_RS19825 3996206..3996547 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
I8N76_RS19830 3996578..3997693 - 1116 WP_198091627.1 MBL fold metallo-hydrolase -
I8N76_RS19835 3997873..3998454 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
I8N76_RS19840 3998454..3998822 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
I8N76_RS19845 3998942..3999595 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
I8N76_RS19850 3999860..4000147 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
I8N76_RS19855 4000140..4000457 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I8N76_RS19860 4000642..4001685 - 1044 WP_048299257.1 DUF2157 domain-containing protein -
I8N76_RS19865 4002351..4003217 - 867 WP_048299254.1 helix-turn-helix transcriptional regulator -
I8N76_RS19870 4003326..4004753 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T183450 WP_004142563.1 NZ_CP065831:c4000457-4000140 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T183450 NZ_CP087020:c52734-52468 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGTTGGTTTTTTCCGATCAGGCTTGGGAAGACTATCAGTACTGGGTCAACACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAGTGCAGGCGGACCCCTTTCAAAGGAACCGGCAAGCCGGAACCTTTGAAGGGCGATCTGACCG
GCTGGTGGTCACGGCGGATTTCTCAGGAAGATCGAATGGTCTACCGGGTTAGTGGAACTGGCGACGGCCAAAGCCTAGAG
ATTGCACAACTGCGGTTTCATTACTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT183450 WP_004142561.1 NZ_CP065831:c4000147-3999860 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT183450 NZ_CP087020:c52982-52731 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTATTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCGATCCACGACAAGCAAGAAGT
TGTTGTGACGCGCAAAAAAGGAGAGTCCGTCGTGGTCGTATCCTTGGAAACCTGGAACGCCGTAAATGAGACGCTGCACC
TCCTGTCCACACCAAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGTGCGTGAG
CTGACCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References