Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 323223..323809 Replicon chromosome
Accession NZ_CP065831
Organism Klebsiella pneumoniae strain KpC11

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag I8N76_RS01505 Protein ID WP_002920800.1
Coordinates 323441..323809 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag I8N76_RS01500 Protein ID WP_004174006.1
Coordinates 323223..323444 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I8N76_RS01480 319380..320306 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
I8N76_RS01485 320303..321580 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
I8N76_RS01490 321577..322344 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
I8N76_RS01495 322346..323059 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
I8N76_RS01500 323223..323444 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
I8N76_RS01505 323441..323809 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
I8N76_RS01510 324082..325398 + 1317 WP_040210732.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
I8N76_RS01515 325505..326392 + 888 WP_069135689.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
I8N76_RS01520 326389..327234 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
I8N76_RS01525 327236..328306 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 320303..329043 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T183436 WP_002920800.1 NZ_CP065831:323441-323809 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T183436 NZ_CP087003:104465-104731 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGCTGGTTTTTTCCGATCAGGCTTGGGAAGACTATCAGTACTGGGTCAACACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAGTGCAGGCGGACCCCTTTCAAAGGAACCGGCAAGCCGGAACCTTTGAAGGGCGATCTGACCG
GCTGGTGGTCACGGCGGATTTCTCAGGAAGATCGAATGGTCTACCGGGTTAGTGGAACTGGCGACGGCCAAAGCCTAGAG
ATTGCACAACTGCGGTTTCATTACTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT183436 WP_004174006.1 NZ_CP065831:323223-323444 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT183436 NZ_CP087003:104217-104468 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTATTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCGATCCACGACAAGCAAGAAGT
TGTTGTGACGCGCAAGAAAGGAGAGTCCGTCGTGGTCGTATCCTTGGAAACCTGGAACGCCGTAAATGAGACGCTGCACC
TCCTGTCCACACCGAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGTGCGTGAG
CTGACCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References