Detailed information of TA system TA183318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4953172..4953795
• Replicon: chromosome
• Accession: NZ_CP065748
• Organism: Delftia lacustris strain FDAARGOS_890

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7T3DCK5
• Locus tag: I6G47_RS23030
• Protein ID: WP_016452144.1
• Coordinates: 4953172..4953453 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6G47_RS23035
• Protein ID: WP_013800141.1
• Coordinates: 4953496..4953795 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G47_RS23005	4948528..4949391	+	864	WP_016448674.1	branched-chain amino acid ABC transporter permease	-
I6G47_RS23010	4949392..4950366	+	975	WP_016452147.1	branched-chain amino acid ABC transporter permease	-
I6G47_RS23015	4950353..4951117	+	765	WP_016452146.1	ABC transporter ATP-binding protein	-
I6G47_RS23020	4951104..4951811	+	708	WP_016448672.1	ABC transporter ATP-binding protein	-
I6G47_RS23025	4951867..4953042	+	1176	WP_016452145.1	ABC transporter substrate-binding protein	-
I6G47_RS23030	4953172..4953453	+	282	WP_016452144.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6G47_RS23035	4953496..4953795	+	300	WP_013800141.1	HigA family addiction module antidote protein	Antitoxin
I6G47_RS23040	4953888..4954661	-	774	WP_016452143.1	SDR family oxidoreductase	-
I6G47_RS23045	4954797..4955603	-	807	WP_016448668.1	IclR family transcriptional regulator	-
I6G47_RS23050	4955648..4956421	-	774	WP_016448667.1	ferredoxin--NADP reductase	-
I6G47_RS23055	4956683..4957618	-	936	WP_016452142.1	LysR family transcriptional regulator	-
I6G47_RS23060	4957711..4958517	+	807	WP_016452141.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4930025..5107108	177083

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.72 Da
• Isoelectric Point: 10.1059

>T183318 WP_016452144.1 NZ_CP065748:4953172-4953453 [Delftia lacustris]
MTIQSFKCPDTEQLFQQRRVRRWSSIEVVALRKLRMLHAAHVLQDLRVPPGNRLEALHGDRKGQHSIRINDQWRVCFVWT
HEGPSQLEIVDYH

Nucleotide

• Length: 282 bp

>T183318 NZ_CP086888:92529-92780 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTATTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCGATCCACGACAAGCAAGAAGT
TGTTGTGACGCGCAAAAAAGGAGAGTCCGTCGTGGTCGTATCCTTGGAAACCTGGAACGCCGTAAATGAGACGCTGCACC
TCCTGTCCACACCAAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGTGCGTGAG
CTGACCGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10990.57 Da
• Isoelectric Point: 4.5183

>AT183318 WP_013800141.1 NZ_CP065748:4953496-4953795 [Delftia lacustris]
MNKPLNLPTPGEILATEFLEPMGLTQYRLAQAIHVPQTRIAAIVKNGRSITADTALRLARFFGTSVEFWMNLQSQYDADI
AREAIGDDLESIEPFALQA

Nucleotide

• Length: 300 bp

>AT183318 NZ_CP086888:92777-93043 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGTTGGTTTTTTCCGATCAGGCTTGGGAAGACTATCAGTACTGGGTCAACACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAGTGCAGGCGGACCCCTTTCAAAGGAACCGGCAAGCCGGAACCTTTGAAGGGCGATCTGACCG
GCTGGTGGTCACGGCGGATTTCTCAGGAAGATCGAATGGTCTACCGGGTTAGTGGAACTGGCGACGGCCAAAGCCTAGAG
ATTGCACAACTGCGGTTTCATTACTGA

Structures

Toxin

• AlphaFold DB: A0A7T3DCK5