Detailed information of TA system TA183297

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1129803..1130455
• Replicon: chromosome
• Accession: NZ_CP065747
• Organism: Serratia plymuthica strain FDAARGOS_896

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I6G53_RS05040
• Protein ID: WP_062868391.1
• Coordinates: 1130111..1130455 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2X4V2M0
• Locus tag: I6G53_RS05035
• Protein ID: WP_063200237.1
• Coordinates: 1129803..1130105 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G53_RS05015	1125081..1125989	+	909	WP_004952315.1	LysR family transcriptional regulator	-
I6G53_RS05020	1126306..1127460	+	1155	WP_004952311.1	MFS transporter	-
I6G53_RS05025	1127450..1128373	-	924	WP_004952309.1	LysR family transcriptional regulator	-
I6G53_RS05030	1128510..1129772	+	1263	WP_197929804.1	diaminopimelate decarboxylase	-
I6G53_RS05035	1129803..1130105	-	303	WP_063200237.1	helix-turn-helix transcriptional regulator	Antitoxin
I6G53_RS05040	1130111..1130455	-	345	WP_062868391.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6G53_RS05045	1130594..1131604	-	1011	WP_062868390.1	LacI family DNA-binding transcriptional regulator	-
I6G53_RS05050	1131756..1132211	+	456	WP_197929812.1	GNAT family N-acetyltransferase	-
I6G53_RS05055	1132208..1133227	-	1020	WP_004952299.1	HTH-type transcriptional regulator GalR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-85)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13304.36 Da
• Isoelectric Point: 10.0970

>T183297 WP_062868391.1 NZ_CP065747:c1130455-1130111 [Serratia plymuthica]
MWQVVTVERFDDWFLSLNGDEQKSLLAGIFKLQEFGPLLARPHADTLHFSGSIKHLKELRIQHRGRPFRVFFAFDPQRQA
VLLCGGDKTGDKRFYQRLLPIAAMEFSHYLATQR

Nucleotide

• Length: 345 bp

>T183297 NZ_CP086866:c28019-27624 [Pseudosulfitobacter pseudonitzschiae]
ATGAGCGTTGAGTTCGCGGACACGAATGTCGTTCTTTACCTGCTCGACGACGGCCCAAAGGCCGATCGCGCCGAGGTCAT
CCTGGGGCAGGGGCCCCGGATCAGCGTTCAGGTTCTAAACGAGTCACTGGTGAACTGCCGCCGCAAAGCTGGCCTCGGTT
GGGAGGAAGCAGCGGCCTTTCTCGAAGGCGTGCGCGCCTTGTGTCCCGTCGAAGATCTCACCGTGCAGACCCATGACGTC
GGCCGCGCTTTGGCGGAACGCTACGGCTTCTCGATCTACGACGCGATGATCGTGGCCAGCGCTTTGGTTGCGGGGTGTAC
CACGCTGTGGAGCGAGGACATGCAAGATGGCCTGCTGGTGGAAGGCCAGCTTCGCATCGTCAACCCCTTTGCATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11126.05 Da
• Isoelectric Point: 10.1425

>AT183297 WP_063200237.1 NZ_CP065747:c1130105-1129803 [Serratia plymuthica]
MAKPLSNLIDKLDPTVVAAARQKAEKEIFELRLAMLREELAVSQVELAKRLGISQPSVANLEKRGSEIKLSSLKRYIEAM
GGTLSLEVELPNGQHRRMTL

Nucleotide

• Length: 303 bp

>AT183297 NZ_CP086866:c28236-28009 [Pseudosulfitobacter pseudonitzschiae]
ATGCAGGTCGCAAAATGGGGTAACTCGCTGGCCGTCCGTTTGCCCGCGGAGCTCGTCCGAGAGCTTGGTCTCAAGGAGGG
TGATCAGATCGACCTGGTCAAGGACGACGGTCGGGTCAGAGTCCGTCGCCTTGCTCGTGCGGATGAGGTACTTACCGGCC
TGCGCCGCTTCCGGGGCAAGTTGTCCGCAGCAGAACGTCTGAGCCGCGACGACGCACATGAGCGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2X4V2M0