Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1275617..1276184 Replicon chromosome
Accession NZ_CP065725
Organism Oligella ureolytica strain FDAARGOS_872

Toxin (Protein)


Gene name higB Uniprot ID A0A095YVF3
Locus tag I6G29_RS06405 Protein ID WP_018027459.1
Coordinates 1275906..1276184 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A378XEC8
Locus tag I6G29_RS06400 Protein ID WP_018575635.1
Coordinates 1275617..1275895 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6G29_RS06370 1271833..1272189 + 357 WP_115148548.1 ATP-binding protein -
I6G29_RS06375 1272446..1273456 - 1011 WP_115061735.1 IS5 family transposase -
I6G29_RS06380 1273681..1274691 - 1011 WP_115061735.1 IS5 family transposase -
I6G29_RS06385 1274880..1275086 + 207 WP_197933430.1 hypothetical protein -
I6G29_RS06390 1275109..1275306 - 198 WP_018575637.1 hypothetical protein -
I6G29_RS06395 1275296..1275514 - 219 WP_018575636.1 TraY domain-containing protein -
I6G29_RS06400 1275617..1275895 - 279 WP_018575635.1 HigA family addiction module antidote protein Antitoxin
I6G29_RS06405 1275906..1276184 - 279 WP_018027459.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6G29_RS06410 1276370..1277514 + 1145 WP_115062989.1 IS3 family transposase -
I6G29_RS06415 1277790..1279009 + 1220 WP_115061740.1 IS3 family transposase -
I6G29_RS06420 1279368..1279805 - 438 WP_018575206.1 tyrosine-type recombinase/integrase -
I6G29_RS06425 1279860..1280645 - 786 WP_018575205.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn blaCARB-5 - 1263226..1279009 15783


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(3-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(10-59)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10814.33 Da        Isoelectric Point: 9.9606

>T183284 WP_018027459.1 NZ_CP065725:c1276184-1275906 [Oligella ureolytica]
MIKSFKHKGLRKYFETGNTSGIQPKHAVRLRTQLSVLDTIKSPDEINIVSWRLHALTGNLKGYWSITVNANWRITFRFEG
ENVYEVDYLDYH

Download         Length: 279 bp

>T183284 NZ_CP086854:3658613-3658876 [Pseudosulfitobacter pseudonitzschiae]
ATGAAGTTCGAGTATGACCCTGACAAGAGTGCCGGGAACAAGGCCAAGCACGGCATCGACTTCGATGAAGCGCAAGCGCT
TTGGGAAGACCCGTGGCTGTTGGAGGCTCCCGCTCGAACAGATGACGAGCCGCGCTTTTTTGCAATAGGCATGATCAAGG
AACGGCATTGGTCAGCAGTTTGCACCTATCGCGGTGATATTATTCGGATCATCTCGGTGCGCCGCGCCAGAAAACAGGAG
ATTGAGCACTATGAAAGCACGTGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10358.89 Da        Isoelectric Point: 5.6491

>AT183284 WP_018575635.1 NZ_CP065725:c1275895-1275617 [Oligella ureolytica]
MRMYNPPHPGLVIREYLNDVTVSDAAKHLGITRTSLSRILNGKTGISPLMAIKLEKAFGGITAESWLAMQAKYDLWQEEQ
SYDDTVIPFEFA

Download         Length: 279 bp

>AT183284 NZ_CP086854:3658863-3659078 [Pseudosulfitobacter pseudonitzschiae]
ATGAAAGCACGTGAGTTCGATCAAAAATTTGACACAGGTGAGAACGTCAGCGATCTTGTGGACTGGTCCAAAGCTAGGCG
GACGAATACCCAGACGAAGCGAGTCAATGTGGATTTTCCGTCGTGGGTCGTTTCTGGTTTGGATCAGAAGGCAAAGCAGC
TCGGCGTTACTCGTCAGTCGCTCATCAAACTCTGGATCGCCGAGCGGCTCCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A095YVF3

Antitoxin

Source ID Structure
AlphaFold DB A0A378XEC8

References