Detailed information of TA system TA183279

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2027309..2027919
• Replicon: chromosome
• Accession: NZ_CP065721
• Organism: Pseudomonas stutzeri strain FDAARGOS_876

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A482U652
• Locus tag: I6G33_RS09530
• Protein ID: WP_017244792.1
• Coordinates: 2027605..2027919 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6G33_RS09525
• Protein ID: WP_003286995.1
• Coordinates: 2027309..2027599 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G33_RS09505	2023270..2023761	+	492	WP_058147936.1	phosphate-starvation-inducible PsiE family protein	-
I6G33_RS09510	2023739..2024704	+	966	WP_058147937.1	M48 family metalloprotease	-
I6G33_RS09515	2024729..2025880	+	1152	WP_058147938.1	trypsin-like peptidase domain-containing protein	-
I6G33_RS09520	2026114..2027022	+	909	WP_003141085.1	LysR family transcriptional regulator	-
I6G33_RS09525	2027309..2027599	-	291	WP_003286995.1	helix-turn-helix domain-containing protein	Antitoxin
I6G33_RS09530	2027605..2027919	-	315	WP_017244792.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6G33_RS09535	2027964..2028989	-	1026	WP_102827463.1	tyrosine-type recombinase/integrase	-
I6G33_RS09540	2028971..2030248	-	1278	WP_102827464.1	hypothetical protein	-
I6G33_RS09545	2030392..2031576	-	1185	WP_102827465.1	zonular occludens toxin	-
I6G33_RS09550	2031589..2031915	-	327	WP_102827466.1	DUF2523 domain-containing protein	-
I6G33_RS09555	2031915..2032214	-	300	WP_102827736.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2026114..2038636	12522

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11953.71 Da
• Isoelectric Point: 9.8035

>T183279 WP_017244792.1 NZ_CP065721:c2027919-2027605 [Pseudomonas stutzeri]
MIFIETPIFTKRLRDLLSDDSYAEFQRQLADRPDMGDVIEGTGGIRKVRVASSGHGKRGGSRVIYYHFTAASQIALLLIY
PKNEKDDLTADERKVLKQIIERWR

Nucleotide

• Length: 315 bp

>T183279 NZ_CP086851:c227563-227297 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGTTGGTTTTTTCCGATCAGGCTTGGGAAGACTATCAGTACTGGGTCAACACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAGTGCAGGCGGACCCCTTTCAAAGGAACCGGCAAGCCGGAACCTTTGAAGGGCGATCTGACCG
GCTGGTGGTCACGGCGGATTTCTCAGGAAGATCGAATGGTCTACCGGGTTAGTGGAACTGGCGACGGCCAAAGCCTAGAG
ATTGCACAACTGCGGTTTCATTACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10930.53 Da
• Isoelectric Point: 10.0845

>AT183279 WP_003286995.1 NZ_CP065721:c2027599-2027309 [Pseudomonas stutzeri]
MSKFFEDLLESVQQMDEIRRGERQPSREFTVDALQVKEIRKATGLTQAKFAAMIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPKHVIQALSSV

Nucleotide

• Length: 291 bp

>AT183279 NZ_CP086851:c227811-227560 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTATTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCGATCCACGACAAGCAAGAAGT
TGTTGTGACGCGCAAAAAAGGAGAGTCCGTCGTGGTCGTATCCTTGGAAACCTGGAACGCCGTAAATGAGACGCTGCACC
TCCTGTCCACACCAAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGTGCGTGAG
CTGACCGAGTGA

Structures

Toxin

• AlphaFold DB: A0A482U652