Detailed information of TA system TA183217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2021509..2022180
• Replicon: chromosome
• Accession: NZ_CP065695
• Organism: Delftia acidovorans strain FDAARGOS_891

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A543BG95
• Locus tag: I6G48_RS09425
• Protein ID: WP_012203142.1
• Coordinates: 2021509..2021790 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: I6G48_RS09430
• Protein ID: WP_012203141.1
• Coordinates: 2021869..2022180 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G48_RS09405	2017201..2017767	-	567	WP_043783206.1	type I-F CRISPR-associated endoribonuclease Cas6/Csy4	-
I6G48_RS09410	2017773..2018801	-	1029	WP_012203145.1	type I-F CRISPR-associated protein Csy3	-
I6G48_RS09415	2018856..2019944	-	1089	WP_012203144.1	type I-F CRISPR-associated protein Csy2	-
I6G48_RS09420	2019941..2021338	-	1398	WP_012203143.1	type I-F CRISPR-associated protein Csy1	-
I6G48_RS09425	2021509..2021790	+	282	WP_012203142.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6G48_RS09430	2021869..2022180	+	312	WP_012203141.1	HigA family addiction module antidote protein	Antitoxin
I6G48_RS09435	2022184..2025670	-	3487	Protein_1856	type I-F CRISPR-associated helicase Cas3	-
I6G48_RS09440	2025667..2026680	-	1014	WP_012203140.1	type I-F CRISPR-associated endonuclease Cas1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-98)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10679.22 Da
• Isoelectric Point: 8.5442

>T183217 WP_012203142.1 NZ_CP065695:2021509-2021790 [Delftia acidovorans]
MAITSFKCPETQRLFTSGKTRRFANIQSVAERKLAQLDAAVTLEFLRSPPGNRLEMLVGDRQGQHSIRINDQWRVCFVWT
EKGVADVEIVDYH

Nucleotide

• Length: 282 bp

>T183217 NZ_CP086790:c2524162-2523908 [Pseudosulfitobacter pseudonitzschiae]
ATGAACAGCAAGCAGCGGAAAACACTCAAGGCGATTTTCACCGATCCGGTGTCTGGCACAATCGAATGGGTCAGGATCGA
AAGTCTCCTTGTCGCGGCTGGCGCTGAAACCATCGAAGGCAACGGCTCGCGCGTTCGCTTTGTTAAGGACGGCGTGGTTG
GATCGTTCCACCGCCCGCACCCTGACAAAGAGGCAAAGCGCTATCAGGTTAGAGACGCCCGCGCCTTCCTAACGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11615.32 Da
• Isoelectric Point: 8.6927

>AT183217 WP_012203141.1 NZ_CP065695:2021869-2022180 [Delftia acidovorans]
MLKNGMRPIHPGEVLREDYLKPMDMSANALARHLHVPASRVNDIVLERRGITADTALRLSRFFGGDAQSWLNLQTAYDLR
TAELNAELQASVRQVHPMQLHTA

Nucleotide

• Length: 312 bp

>AT183217 NZ_CP086790:c2523911-2523582 [Pseudosulfitobacter pseudonitzschiae]
ATGAGCACCATGACTTTCAAAGGCTATCAGGCCCGTGTGGAGTTCGATCCAGAGGACGAGATTTTCTTCGGTAGAATTGC
CGGGATAAACGATGTGGTTGGCTTCCACGGCGAAAGCGTTGCCGAGCTTAAGAGTGCCTTTCATGAAGCCGTTGATGATT
ATCTTGAAACCTGCAAGGCGGCTGGCAAGAAGCCCCAGAAGCCGTATTCTGGCAAGGTGATGTTTCGAGTCGCTCCTGAA
ACCCACAGGCGCATCGCAGTTGCAGCTGAACTTTCCGGCAAGAGCCTGAACCAGTGGGCAGAAGAAAAGCTAGAGGCTGC
TGCCCACTAA

Structures

Toxin

• AlphaFold DB: A0A543BG95