Detailed information of TA system TA183202

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 836374..837104
• Replicon: chromosome
• Accession: NZ_CP065693
• Organism: Enterobacter asburiae strain FDAARGOS_892

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A376FEI5
• Locus tag: I6G49_RS04280
• Protein ID: WP_029741423.1
• Coordinates: 836790..837104 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6G49_RS04275
• Protein ID: WP_167347079.1
• Coordinates: 836374..836793 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G49_RS04250	831846..833351	-	1506	WP_059347367.1	signal transduction histidine-protein kinase/phosphatase UhpB	-
I6G49_RS04255	833348..833941	-	594	WP_029741420.1	transcriptional regulator UhpA	-
I6G49_RS04260	834042..834329	-	288	WP_010426528.1	acetolactate synthase small subunit	-
I6G49_RS04265	834333..836021	-	1689	WP_059347368.1	acetolactate synthase large subunit	-
I6G49_RS04270	836128..836226	-	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
I6G49_RS04275	836374..836793	-	420	WP_167347079.1	helix-turn-helix domain-containing protein	Antitoxin
I6G49_RS04280	836790..837104	-	315	WP_029741423.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6G49_RS04285	837591..837680	+	90	WP_023309830.1	type I toxin-antitoxin system toxin TisB	-
I6G49_RS04290	837745..838191	+	447	WP_059347369.1	GNAT family N-acetyltransferase	-
I6G49_RS04295	838254..839087	+	834	WP_059347370.1	DMT family transporter	-
I6G49_RS04300	839263..840447	+	1185	WP_029741426.1	multidrug efflux MFS transporter EmrD	-
I6G49_RS04305	840350..841438	-	1089	WP_059347371.1	cellulase family glycosylhydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12361.46 Da
• Isoelectric Point: 10.2219

>T183202 WP_029741423.1 NZ_CP065693:c837104-836790 [Enterobacter asburiae]
MHLISMKAILDAVTQFPQHKDELLFLGRTIEKSHCPTPAALKKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T183202 NZ_CP086774:34937-35203 [Pseudosulfitobacter pseudonitzschiae]
GTGAAGCTGGTTTTTTCGGATCAGGCCTGGGAAGATTATCAGTACTGGGTCAGCACCAACGACAAGGTACGTGACCGGAT
CAACGAGCTGATCAAGCAATGCAAACGGACCCCCTTCAAAGGTTCCGGCTTGCCGGAACCTTTGAAGGGGGATTTGACAG
GCTGGTGGTCACGGCGAATTTCTCAGGAAGATCGAATGGTTTACCGGGTCAGTGGAACTGGCGACGGCCAAAGCTTAGAG
ATTGCGCAACTTCGGTTTCATTACTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15487.99 Da
• Isoelectric Point: 4.5348

>AT183202 WP_167347079.1 NZ_CP065693:c836793-836374 [Enterobacter asburiae]
MMIVADAMKATHALVAAVPLLGEQPSEKDYEDALELVEYLLMNEPDSPLLDIVCARISRYEANRPDIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFEDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT183202 NZ_CP086774:34689-34940 [Pseudosulfitobacter pseudonitzschiae]
ATGGATGTTATGACCTACTCAGACGCACGAGCTCAACTGAAGGGCGTCATGGATCGAGCTATCCACGACAAGCAGGAAGT
TGTCGTGACGCGCAAGAAGGGCGAGTCCGTTGTGGTCGTGTCCTTGGACACTTGGAACGCCGTCAACGAGACTTTGCACC
TGTTGTCCACACCGAAAAACGCATCCCGCTTGCGCGCGTCGATCGCGCAACTCGATGCTGGAACTGGCGAAGAGCGTGAG
CTGACCGAGTGA

Structures

Toxin

• AlphaFold DB: A0A376FEI5