Detailed information of TA system TA183177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3989847..3990504
• Replicon: chromosome
• Accession: NZ_CP065686
• Organism: Burkholderia humptydooensis strain FDAARGOS_899

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7U4P172
• Locus tag: I6G56_RS19725
• Protein ID: WP_009913956.1
• Coordinates: 3990172..3990504 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6G56_RS19720
• Protein ID: WP_009913954.1
• Coordinates: 3989847..3990164 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G56_RS19695	3985806..3986090	-	285	WP_006023861.1	type II toxin-antitoxin system RelE/ParE family toxin	-
I6G56_RS19700	3986087..3986551	-	465	WP_043281857.1	hypothetical protein	-
I6G56_RS19705	3986697..3987458	-	762	WP_043281860.1	NYN domain-containing protein	-
I6G56_RS19710	3987696..3987929	-	234	WP_009913949.1	hypothetical protein	-
I6G56_RS19715	3988922..3989803	-	882	WP_158354520.1	DUF4935 domain-containing protein	-
I6G56_RS19720	3989847..3990164	-	318	WP_009913954.1	helix-turn-helix domain-containing protein	Antitoxin
I6G56_RS19725	3990172..3990504	-	333	WP_009913956.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6G56_RS19730	3990545..3991132	-	588	WP_043282124.1	site-specific integrase	-
I6G56_RS19735	3991499..3992278	-	780	WP_043281862.1	hypothetical protein	-
I6G56_RS19740	3992723..3993304	-	582	WP_009913959.1	hypothetical protein	-
I6G56_RS19745	3993404..3994774	-	1371	WP_043281864.1	type II secretory pathway protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3980803..4002033	21230

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-100)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12677.69 Da
• Isoelectric Point: 10.4257

>T183177 WP_009913956.1 NZ_CP065686:c3990504-3990172 [Burkholderia humptydooensis]
MTQNWTIVYYNERVKRDVFALPAGILADYLRLLDLMQEFGADLRMPHSRAMGGGLFELRPKGREGIGRVFYCTHVGQRVV
VLHSFVKKTQETPQNELRTARVRLSEVRNG

Nucleotide

• Length: 333 bp

>T183177 NZ_CP086759:1432980-1433267 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11340.91 Da
• Isoelectric Point: 8.0784

>AT183177 WP_009913954.1 NZ_CP065686:c3990164-3989847 [Burkholderia humptydooensis]
VKRASAEGFNPIQHTVDDTEHLLAKPGVRVAYDALEDEYMALRAILAIRHEAGLTQAQVAERMGTTASAVSRLEASLSSE
KHSPSFATLRKYAAACGKRLVISFA

Nucleotide

• Length: 318 bp

>AT183177 NZ_CP086759:1433254-1433568 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A7U4P172