Detailed information of TA system TA183172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4139919..4140514
• Replicon: chromosome
• Accession: NZ_CP065683
• Organism: Burkholderia oklahomensis strain FDAARGOS_900

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: I6G57_RS18790
• Protein ID: WP_010111175.1
• Coordinates: 4139919..4140197 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6G57_RS18795
• Protein ID: WP_010111178.1
• Coordinates: 4140215..4140514 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G57_RS18760	4135130..4135459	-	330	WP_159086819.1	IS66 family insertion sequence element accessory protein TnpB	-
I6G57_RS18765	4135477..4135917	-	441	WP_081464416.1	hypothetical protein	-
I6G57_RS18770	4136035..4136877	+	843	WP_038802590.1	IS3 family transposase	-
I6G57_RS18775	4137664..4138326	-	663	WP_010111169.1	DUF2612 domain-containing protein	-
I6G57_RS18780	4138326..4139507	-	1182	WP_010111171.1	baseplate J/gp47 family protein	-
I6G57_RS18785	4139504..4139857	-	354	WP_010111173.1	hypothetical protein	-
I6G57_RS18790	4139919..4140197	+	279	WP_010111175.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6G57_RS18795	4140215..4140514	+	300	WP_010111178.1	HigA family addiction module antidote protein	Antitoxin
I6G57_RS18800	4140580..4141318	-	739	Protein_3694	hypothetical protein	-
I6G57_RS18805	4141322..4141699	+	378	WP_010111182.1	hypothetical protein	-
I6G57_RS18810	4141696..4142664	-	969	WP_010111184.1	hypothetical protein	-
I6G57_RS18815	4142708..4143118	-	411	WP_010111186.1	hypothetical protein	-
I6G57_RS18820	4143105..4143449	-	345	WP_144444658.1	hypothetical protein	-
I6G57_RS18825	4143449..4143868	-	420	WP_144444657.1	hypothetical protein	-
I6G57_RS18830	4143931..4144245	-	315	WP_010111189.1	hypothetical protein	-
I6G57_RS18835	4144245..4144820	-	576	WP_010111191.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4123572..4192823	69251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (23-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10677.16 Da
• Isoelectric Point: 10.2114

>T183172 WP_010111175.1 NZ_CP065683:4139919-4140197 [Burkholderia oklahomensis]
MITSFNCRDSEALFNGRRVARFANIERVAIRKLQQLHAAADLDFLRIPPNNRLEALKGDRQGQFSIRINDQWRICFRFAG
GRASDVEIVDYH

Nucleotide

• Length: 279 bp

>T183172 NZ_CP086728:c1342806-1342468 [Streptococcus parasuis]
ATGGAGAAATATAGAGTCATTATTTCGCCAAAGCTTATTGAGCAAATCAAAAGTATTAGGGATTATGTTGCTAATGTGAA
ACTGAGTCCAGAAACTGCAGAGCGTTTAGTAACGGCCATTTTTGATGAAATAGAGAGCCTAGAACTCTTTCCAGAGCGTG
GATTCGATGCTGATGAAAAAATTGGAGTTAAGATTGATAAGAATGGCCGTAGTAGCCGAGGAATAACCATTCAAAATGGA
AAATATATTGTCATCTATACTGTAAACCAGGAAGAGCATTTAGTTGAAGTTGCTTTCCTATTCTCATCAAAGACTGATTA
TGCTAAACTTTTTAGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10953.55 Da
• Isoelectric Point: 5.6088

>AT183172 WP_010111178.1 NZ_CP065683:4140215-4140514 [Burkholderia oklahomensis]
MTREVPLATPGEILALDWLEPMGISQYALAKAISVPARRINEIVKGDRAITADTAVRLGAFFGVDPQSWMNLQTHYDTAL
AKEKIGDDVLREIREHAHA

Nucleotide

• Length: 300 bp

>AT183172 NZ_CP086728:c1343093-1342806 [Streptococcus parasuis]
ATGCCAACAGTAATAAGGGATAAACAAATCAACTTCAAAGTAAATTCAGAATACTTTGAATTAGCGAAAGTGGTCTTTCA
GGAAAACGGACTGGATATTACATCAGCATTCAATGAATTTGTACAAGAGGTCGCAATTTCCAAAACACTGCCTTTTAAGA
CAGTAGAGGAAAAGGAGAGAGAGAAGTTAATTGCTCGACTTCAAGAAGAAGTTTCCACTAGCTATGAAGCGCTAAAAGCA
GATAAAGGGTTGTCGGTTGAGGAAGCGAGAAAGGCAGTACTAGGATAA