Detailed information of TA system TA183143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 4595966..4596589
• Replicon: chromosome
• Accession: NZ_CP065668
• Organism: Delftia acidovorans strain FDAARGOS_909

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: I6G66_RS21610
• Protein ID: WP_197954472.1
• Coordinates: 4595966..4596247 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6G66_RS21615
• Protein ID: WP_013800141.1
• Coordinates: 4596290..4596589 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G66_RS21585	4591200..4592063	+	864	WP_183021245.1	branched-chain amino acid ABC transporter permease	-
I6G66_RS21590	4592064..4593038	+	975	WP_012202200.1	branched-chain amino acid ABC transporter permease	-
I6G66_RS21595	4593025..4593789	+	765	WP_013800137.1	ABC transporter ATP-binding protein	-
I6G66_RS21600	4593776..4594483	+	708	WP_183021247.1	ABC transporter ATP-binding protein	-
I6G66_RS21605	4594661..4595836	+	1176	WP_183021248.1	ABC transporter substrate-binding protein	-
I6G66_RS21610	4595966..4596247	+	282	WP_197954472.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6G66_RS21615	4596290..4596589	+	300	WP_013800141.1	HigA family addiction module antidote protein	Antitoxin
I6G66_RS21620	4596682..4597455	-	774	WP_183021249.1	SDR family oxidoreductase	-
I6G66_RS21625	4597581..4598387	-	807	WP_197954473.1	IclR family transcriptional regulator	-
I6G66_RS21630	4598432..4599205	-	774	WP_183021251.1	ferredoxin--NADP reductase	-
I6G66_RS21635	4599466..4600401	-	936	WP_197954474.1	LysR family transcriptional regulator	-
I6G66_RS21640	4600495..4601286	+	792	WP_097204607.1	SDR family oxidoreductase	-
I6G66_RS21645	4601304..4601585	-	282	WP_183021253.1	DUF3325 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11095.76 Da
• Isoelectric Point: 10.1036

>T183143 WP_197954472.1 NZ_CP065668:4595966-4596247 [Delftia acidovorans]
MTIRSFKCPDTEQLFQQRRVRRWSSIEVVALRKLRMLHAAHVLQDLRVPPGNRLEALHGDRKGQHSIRINDQWRVCFVWT
HEGPSELEIVDYH

Nucleotide

• Length: 282 bp

>T183143 NZ_CP086681:22303-22683 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10990.57 Da
• Isoelectric Point: 4.5183

>AT183143 WP_013800141.1 NZ_CP065668:4596290-4596589 [Delftia acidovorans]
MNKPLNLPTPGEILATEFLEPMGLTQYRLAQAIHVPQTRIAAIVKNGRSITADTALRLARFFGTSVEFWMNLQSQYDADI
AREAIGDDLESIEPFALQA

Nucleotide

• Length: 300 bp

>AT183143 NZ_CP086681:22082-22303 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA