Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4901733..4902326 Replicon chromosome
Accession NZ_CP065627
Organism Delftia acidovorans strain FDAARGOS_939

Toxin (Protein)


Gene name higB Uniprot ID F6ASE3
Locus tag I6G96_RS22240 Protein ID WP_013802475.1
Coordinates 4901733..4902011 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1C7LFY9
Locus tag I6G96_RS22245 Protein ID WP_063326188.1
Coordinates 4902024..4902326 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6G96_RS22215 4897000..4897707 - 708 WP_197943379.1 fumarylacetoacetate hydrolase family protein -
I6G96_RS22220 4897746..4898783 - 1038 WP_197943380.1 cupin domain-containing protein -
I6G96_RS22225 4898800..4900008 - 1209 WP_197943381.1 3-hydroxybenzoate 6-monooxygenase -
I6G96_RS22230 4900213..4900749 + 537 WP_180317110.1 MarR family winged helix-turn-helix transcriptional regulator -
I6G96_RS22235 4900764..4901666 + 903 WP_013802476.1 LysR family transcriptional regulator -
I6G96_RS22240 4901733..4902011 + 279 WP_013802475.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6G96_RS22245 4902024..4902326 + 303 WP_063326188.1 HigA family addiction module antidote protein Antitoxin
I6G96_RS22250 4902441..4903499 + 1059 WP_197943382.1 patatin-like phospholipase family protein -
I6G96_RS22255 4903521..4904795 - 1275 WP_197943383.1 serine hydroxymethyltransferase -
I6G96_RS22260 4904842..4906068 - 1227 WP_197943384.1 aspartate aminotransferase family protein -
I6G96_RS22265 4906138..4906989 - 852 WP_197943385.1 YggS family pyridoxal phosphate-dependent enzyme -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(17-66)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10518.17 Da        Isoelectric Point: 9.2618

>T183095 WP_013802475.1 NZ_CP065627:4901733-4902011 [Delftia acidovorans]
MILDFRCADTQALMLGKRVARFANLEAAAMRKLVQLNAATSLEFLRIPPGNRLEALQGSRRGQYSIRINEQWRLCFVWAD
GHASQVEIVDYH

Download         Length: 279 bp

>T183095 NZ_CP086662:3482307-3482480 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11373.07 Da        Isoelectric Point: 5.6088

>AT183095 WP_063326188.1 NZ_CP065627:4902024-4902326 [Delftia acidovorans]
MTREVKWPHPGEILMHEWLEPMGLSQYALAKAIDVPPRRINEIVKGMRGITADTALRLAVFFGTDAQSWLNLQSDYDLAE
ARDAMADVLARIQRFEPSHP

Download         Length: 303 bp

>AT183095 NZ_CP086662:3481983-3482279 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A031I1T8

Antitoxin

Source ID Structure
AlphaFold DB A0A1C7LFY9

References