Detailed information of TA system TA183070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2397262..2397864
• Replicon: chromosome
• Accession: NZ_CP065624
• Organism: Escherichia coli strain FDAARGOS_941

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: I6G98_RS12815
• Protein ID: WP_000897305.1
• Coordinates: 2397262..2397573 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6G98_RS12820
• Protein ID: WP_000356397.1
• Coordinates: 2397574..2397864 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G98_RS12795	2392305..2392742	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
I6G98_RS12800	2392787..2393728	+	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
I6G98_RS12805	2394143..2395957	+	1815	WP_032360144.1	hypothetical protein	-
I6G98_RS12810	2396125..2397033	-	909	WP_032360143.1	alpha/beta hydrolase	-
I6G98_RS12815	2397262..2397573	+	312	WP_000897305.1	hypothetical protein	Toxin
I6G98_RS12820	2397574..2397864	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
I6G98_RS12825	2398450..2398668	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
I6G98_RS12830	2398887..2399129	+	243	WP_001086388.1	hypothetical protein	-
I6G98_RS12835	2399459..2400388	-	930	WP_032359289.1	formate dehydrogenase accessory protein FdhE	-
I6G98_RS12840	2400385..2401020	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
I6G98_RS12845	2401017..2401919	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T183070 WP_000897305.1 NZ_CP065624:2397262-2397573 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T183070 NZ_CP086654:727443-727667 [Staphylococcus ratti]
ATGTATAAAGATATTGTTTTTGTAGAAGGTATTCAGTCCGATTTACAAAAAGAAAATTTAGCACAACGTTTGAATCAAAT
GATAGGTGTTAAAGAAGTAACGATAGATATTGAACGCCAATCTATTACATTGCTTTATAATACACCTGTGAATTTGAATA
CATTAGAAAAAGAAATATACGATACTGGATACAAAGTGATTCGTACAGAAAAAGGAGCGATTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT183070 WP_000356397.1 NZ_CP065624:2397574-2397864 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT183070 NZ_CP086654:727141-727428 [Staphylococcus ratti]
ATGGAAGAACGTGCACATCATTCAGTGGAAATTAAAAAGAACTTAACCTCACGTTTGAATAGAATAGAAGGACAAGTTCG
TGCGATTAATCGTATGATTGATGAAGATGTCTATTGTGATGATGTATTAACTCAGATTCGAGCTACACGCTCTGCCTTAA
ATAGTGTAGCGACTAAATTATTGGATTATCACATGAAAGGCTGTATTACTCAAAAAATTGAAGAGGGCCATGAGACAGAA
GCCATGGAGGAGCTACTCGTTACTTTTCAAAAATTATTAAAAGATTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7