Detailed information of TA system TA183068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1608137..1608936
• Replicon: chromosome
• Accession: NZ_CP065624
• Organism: Escherichia coli strain FDAARGOS_941

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A3I9P420
• Locus tag: I6G98_RS08990
• Protein ID: WP_000347271.1
• Coordinates: 1608472..1608936 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: I6G98_RS08985
• Protein ID: WP_001302819.1
• Coordinates: 1608137..1608472 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G98_RS08970	1603921..1604691	-	771	WP_001058214.1	2-dehydro-3-deoxyglucarate aldolase	-
I6G98_RS08975	1604707..1606002	-	1296	WP_197932984.1	MFS transporter	-
I6G98_RS08980	1606417..1607988	+	1572	WP_001273770.1	galactarate dehydratase	-
I6G98_RS08985	1608137..1608472	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
I6G98_RS08990	1608472..1608936	+	465	WP_000347271.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
I6G98_RS08995	1608991..1609800	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
I6G98_RS09000	1610049..1611329	+	1281	WP_032359524.1	tagatose-bisphosphate aldolase subunit KbaZ	-
I6G98_RS09005	1611352..1611825	+	474	WP_016245132.1	PTS N-acetylgalactosamine transporter subunit IIB	-
I6G98_RS09010	1611836..1612615	+	780	WP_197932950.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
I6G98_RS09015	1612605..1613483	+	879	WP_001380079.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
I6G98_RS09020	1613501..1613935	+	435	WP_000948814.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1597507..1608936	11429

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17849.25 Da
• Isoelectric Point: 9.6924

>T183068 WP_000347271.1 NZ_CP065624:1608472-1608936 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEENH

Nucleotide

• Length: 465 bp

>T183068 NZ_CP086627:c4702200-4702027 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT183068 WP_001302819.1 NZ_CP065624:1608137-1608472 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT183068 NZ_CP086627:c4702524-4702228 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A3I9P420