Detailed information of TA system TA183064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1103760..1104487
• Replicon: chromosome
• Accession: NZ_CP065624
• Organism: Escherichia coli strain FDAARGOS_941

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: I6G98_RS06450
• Protein ID: WP_000547563.1
• Coordinates: 1104176..1104487 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6G98_RS06445
• Protein ID: WP_000126296.1
• Coordinates: 1103760..1104179 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6G98_RS06425	1098814..1099341	-	528	WP_001078777.1	electron transport protein HydN	-
I6G98_RS06430	1099490..1100500	-	1011	WP_001402444.1	DNA-binding transcriptional regulator AscG	-
I6G98_RS06435	1100750..1102215	+	1466	Protein_1102	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
I6G98_RS06440	1102309..1103646	+	1338	Protein_1103	6-phospho-beta-glucosidase AscB	-
I6G98_RS06445	1103760..1104179	-	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
I6G98_RS06450	1104176..1104487	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6G98_RS06455	1104649..1105419	-	771	WP_197932981.1	hypothetical protein	-
I6G98_RS06460	1105448..1105918	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
I6G98_RS06465	1105911..1106321	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
I6G98_RS06470	1106318..1107085	-	768	WP_073840462.1	formate hydrogenlyase subunit HycG	-
I6G98_RS06475	1107085..1107627	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
I6G98_RS06480	1107637..1109346	-	1710	WP_197932922.1	hydrogenase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T183064 WP_000547563.1 NZ_CP065624:c1104487-1104176 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T183064 NZ_CP086627:c4098456-4097536 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCCGGCAGGTCAAACTGTTGATGGCCGCTCTGCCGTATGTGGCGAAGGAGTCCTGTTT
CGCGTTGAAAGGCGGCACCGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATTGATCTGGCGTACA
AGAGCTTTGCGGATCGCGATACTGATTTGGCGGCTATCAACGACGCTTTGAAGAGGATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATCTGCGCTGCGCTTGATCGTCAGCAC
CCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAGAAACCGGGACTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGCCCCATCGCCGAGTTGCTGGCTCCAAACTGGGATACAGAGCGGATCGCCACGTTGTACGCGC
AGGAATTTTCAGGGATGACGCAACAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATCTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGGTGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTTCTGGCGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT183064 WP_000126296.1 NZ_CP065624:c1104179-1103760 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT183064 NZ_CP086627:c4099225-4098443 [Escherichia coli]
TTGCCAAACTATCTAAACTGGCTGATGCAGCACACGCTACCCGGCCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CATAGATCGTAAGCTCTCTTATCTCTATGTGAAGAATGGCTGGCTCAGGCGGATCGCTCACGGCGTATACTGCCGGACGG
GTCGTGAACCGGATTGGGTGGATGCGGTTTACTGCCTGCAATCGCAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGTCATGCGCACTATACCGAACAGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTTCTGCCTTTGAACAATCCGCAACGTTTGTCACGCTGTATACGTCAGGCCTTACCGCTGATGTAA
CGCCATTTACTACGGAACTGAAAATTGGCGACCGCCAGTTAGCAGGCAGTGTTCCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTAATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGTTGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGCCATTCGGTTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATATTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCCGGCGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCACGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22