Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 2021304..2021931 Replicon chromosome
Accession NZ_CP065615
Organism Escherichia coli strain FDAARGOS_943

Toxin (Protein)


Gene name higB Uniprot ID A0A828BKW8
Locus tag I6H00_RS10400 Protein ID WP_024258354.1
Coordinates 2021304..2021633 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID Q32DW6
Locus tag I6H00_RS10405 Protein ID WP_000147081.1
Coordinates 2021623..2021931 (+) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H00_RS10340 2016309..2016776 - 468 WP_197940026.1 lysis protein -
I6H00_RS10345 2016773..2017270 - 498 WP_197940027.1 lysozyme -
I6H00_RS10350 2017270..2017485 - 216 WP_077123425.1 class II holin family protein -
I6H00_RS10365 2018018..2018641 - 624 WP_001235456.1 antitermination protein -
I6H00_RS10370 2018638..2018826 - 189 WP_109536308.1 protein ninH -
I6H00_RS10375 2018823..2019185 - 363 WP_073840903.1 RusA family crossover junction endodeoxyribonuclease -
I6H00_RS10380 2019182..2019952 - 771 WP_001344365.1 phage antirepressor Ant -
I6H00_RS10385 2019967..2020203 - 237 Protein_2039 Rha family transcriptional regulator -
I6H00_RS10390 2020278..2020982 - 705 WP_073840904.1 ORF6N domain-containing protein -
I6H00_RS10395 2021033..2021258 - 226 Protein_2041 ash family protein -
I6H00_RS10400 2021304..2021633 + 330 WP_024258354.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6H00_RS10405 2021623..2021931 + 309 WP_000147081.1 helix-turn-helix transcriptional regulator Antitoxin
I6H00_RS10410 2021973..2022149 - 177 WP_001254216.1 NinE family protein -
I6H00_RS10415 2022146..2022673 - 528 WP_197940028.1 phage N-6-adenine-methyltransferase -
I6H00_RS10420 2022670..2023071 - 402 WP_197940029.1 recombination protein NinB -
I6H00_RS10425 2023087..2024243 + 1157 WP_085948186.1 IS3-like element IS600 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1988349..2101374 113025


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-97)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12837.87 Da        Isoelectric Point: 9.9966

>T183037 WP_024258354.1 NZ_CP065615:2021304-2021633 [Escherichia coli]
MNYTIEYYSEDVRLEVDRLPLSMRARYQHLVERMKIYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIML
HSFVKKTQKTPPAERKKAETRMKEVKHDW

Download         Length: 330 bp

>T183037 NZ_CP086626:3469487-3469873 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11185.94 Da        Isoelectric Point: 8.5840

>AT183037 WP_000147081.1 NZ_CP065615:2021623-2021931 [Escherichia coli]
MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGISG
PSFATLKKFARATGKKLQIRFV

Download         Length: 309 bp

>AT183037 NZ_CP086626:3469863-3470192 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828BKW8

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TJC1

References