Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3748429..3748970 Replicon chromosome
Accession NZ_CP065613
Organism Escherichia coli strain FDAARGOS_944

Toxin (Protein)


Gene name yafQ Uniprot ID A0A377CPJ1
Locus tag I6H01_RS18125 Protein ID WP_038341163.1
Coordinates 3748429..3748707 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag I6H01_RS18130 Protein ID WP_000729704.1
Coordinates 3748710..3748970 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H01_RS18110 3746011..3746589 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
I6H01_RS18115 3746795..3747562 + 768 WP_000333380.1 class II glutamine amidotransferase -
I6H01_RS18120 3747533..3748273 - 741 WP_038341164.1 murein L,D-transpeptidase -
I6H01_RS18125 3748429..3748707 - 279 WP_038341163.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
I6H01_RS18130 3748710..3748970 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
I6H01_RS18135 3749180..3749929 + 750 WP_024191992.1 C40 family peptidase -
I6H01_RS18140 3750105..3750602 + 498 WP_038341161.1 transposase -
I6H01_RS18145 3750788..3752881 - 2094 WP_021556448.1 flagellar biosynthesis protein FlhA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10848.68 Da        Isoelectric Point: 10.1470

>T183022 WP_038341163.1 NZ_CP065613:c3748707-3748429 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKYMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T183022 NZ_CP086626:262336-262443 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT183022 WP_000729704.1 NZ_CP065613:c3748970-3748710 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT183022 NZ_CP086626:c262278-262224 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377CPJ1

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References