Detailed information of TA system TA183021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3569339..3569922
• Replicon: chromosome
• Accession: NZ_CP065613
• Organism: Escherichia coli strain FDAARGOS_944

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A377CN38
• Locus tag: I6H01_RS17245
• Protein ID: WP_000597786.1
• Coordinates: 3569644..3569922 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6H01_RS17240
• Protein ID: WP_000177706.1
• Coordinates: 3569339..3569644 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H01_RS17190	3564587..3564802	+	216	WP_000067727.1	helix-turn-helix transcriptional regulator	-
I6H01_RS17195	3564922..3565217	+	296	Protein_3347	lambda phage CII family protein	-
I6H01_RS17200	3565250..3566188	+	939	WP_073842031.1	replication protein	-
I6H01_RS17205	3566185..3566886	+	702	WP_073842030.1	Replication protein P	-
I6H01_RS17210	3566883..3567173	+	291	WP_073842029.1	protein ren	-
I6H01_RS17215	3567244..3567522	+	279	WP_001000127.1	hypothetical protein	-
I6H01_RS17220	3567879..3568175	+	297	WP_073842028.1	hypothetical protein	-
I6H01_RS17225	3568132..3568599	+	468	WP_073842027.1	recombination protein NinB	-
I6H01_RS17230	3568592..3569122	+	531	WP_000589785.1	phage N-6-adenine-methyltransferase	-
I6H01_RS17235	3569119..3569295	+	177	WP_001254255.1	NinE family protein	-
I6H01_RS17240	3569339..3569644	-	306	WP_000177706.1	HigA family addiction module antidote protein	Antitoxin
I6H01_RS17245	3569644..3569922	-	279	WP_000597786.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6H01_RS17250	3570259..3570945	+	687	WP_000218025.1	phage antirepressor Ant	-
I6H01_RS17255	3571020..3571256	+	237	Protein_3359	Rha family transcriptional regulator	-
I6H01_RS17260	3571271..3572040	+	770	Protein_3360	phage antirepressor KilAC domain-containing protein	-
I6H01_RS17265	3572037..3572399	+	363	WP_073842066.1	RusA family crossover junction endodeoxyribonuclease	-
I6H01_RS17270	3572396..3572584	+	189	WP_000994516.1	protein ninH	-
I6H01_RS17275	3572581..3573099	+	519	WP_073842065.1	DUF1133 family protein	-
I6H01_RS17290	3573435..3573650	+	216	WP_000839570.1	class II holin family protein	-
I6H01_RS17295	3573650..3574147	+	498	WP_000075178.1	lysozyme RrrD	-
I6H01_RS17300	3574144..3574581	+	438	WP_073842038.1	lysis protein	-
I6H01_RS17305	3574716..3574789	+	74	Protein_3367	Rha family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3552877..3597282	44405

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-65)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10797.11 Da
• Isoelectric Point: 7.3997

>T183021 WP_000597786.1 NZ_CP065613:c3569922-3569644 [Escherichia coli]
MIKSWKHKGLQRFFETGSASGVNPNHVKRLRMRLQVIDQAEIIDDINLAGYRLHQLQGDRADTWSVSVSGNWRITFEFRD
GDAYILNYEDYH

Nucleotide

• Length: 279 bp

>T183021 NZ_CP086626:206651-206836 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11121.76 Da
• Isoelectric Point: 5.0873

>AT183021 WP_000177706.1 NZ_CP065613:c3569644-3569339 [Escherichia coli]
MTMFNPPHPGEIIADILEDQNIGIRELARALDVAPSTVQRLVSGNAAISPEMAVRLAAVLGGTPASWIRLQMAWSLEKAE
REVDISHLSTKYRPEEIQPHP

Nucleotide

• Length: 306 bp

>AT183021 NZ_CP086626:206853-207263 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGTGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A377CN38