Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4276637..4277220 Replicon chromosome
Accession NZ_CP065611
Organism Escherichia coli strain FDAARGOS_945

Toxin (Protein)


Gene name higB Uniprot ID A0A377CN38
Locus tag I6H02_RS21940 Protein ID WP_000597786.1
Coordinates 4276942..4277220 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag I6H02_RS21935 Protein ID WP_000177706.1
Coordinates 4276637..4276942 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H02_RS21910 4271849..4272646 + 798 WP_115264060.1 helix-turn-helix domain-containing protein -
I6H02_RS21915 4272754..4275660 + 2907 WP_038341800.1 toprim domain-containing protein -
I6H02_RS21920 4275735..4276013 + 279 WP_001000126.1 hypothetical protein -
I6H02_RS21925 4276010..4276420 + 411 WP_000814622.1 recombination protein NinB -
I6H02_RS21930 4276417..4276593 + 177 WP_001254255.1 NinE family protein -
I6H02_RS21935 4276637..4276942 - 306 WP_000177706.1 HigA family addiction module antidote protein Antitoxin
I6H02_RS21940 4276942..4277220 - 279 WP_000597786.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6H02_RS21945 4277557..4278243 + 687 WP_000218025.1 phage antirepressor Ant -
I6H02_RS21950 4278318..4278554 + 237 Protein_4141 Rha family transcriptional regulator -
I6H02_RS21955 4278569..4279339 + 771 WP_001344365.1 phage antirepressor Ant -
I6H02_RS21960 4279336..4279698 + 363 WP_001008209.1 RusA family crossover junction endodeoxyribonuclease -
I6H02_RS21965 4279695..4279883 + 189 WP_000994516.1 protein ninH -
I6H02_RS21970 4279880..4280503 + 624 WP_001235456.1 antitermination protein -
I6H02_RS21985 4281036..4281251 + 216 WP_000839570.1 class II holin family protein -
I6H02_RS21990 4281256..4281786 + 531 WP_000193253.1 YdfR family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4255891..4308471 52580


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(3-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(10-65)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10797.11 Da        Isoelectric Point: 7.3997

>T182998 WP_000597786.1 NZ_CP065611:c4277220-4276942 [Escherichia coli]
MIKSWKHKGLQRFFETGSASGVNPNHVKRLRMRLQVIDQAEIIDDINLAGYRLHQLQGDRADTWSVSVSGNWRITFEFRD
GDAYILNYEDYH

Download         Length: 279 bp

>T182998 NZ_CP086625:263592-263699 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11121.76 Da        Isoelectric Point: 5.0873

>AT182998 WP_000177706.1 NZ_CP065611:c4276942-4276637 [Escherichia coli]
MTMFNPPHPGEIIADILEDQNIGIRELARALDVAPSTVQRLVSGNAAISPEMAVRLAAVLGGTPASWIRLQMAWSLEKAE
REVDISHLSTKYRPEEIQPHP

Download         Length: 306 bp

>AT182998 NZ_CP086625:c263543-263480 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377CN38

Antitoxin

Source ID Structure

References