Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3713007..3713548 Replicon chromosome
Accession NZ_CP065611
Organism Escherichia coli strain FDAARGOS_945

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag I6H02_RS19085 Protein ID WP_000615976.1
Coordinates 3713007..3713285 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag I6H02_RS19090 Protein ID WP_000729704.1
Coordinates 3713288..3713548 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H02_RS19070 3710589..3711167 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
I6H02_RS19075 3711373..3712140 + 768 WP_024196817.1 class II glutamine amidotransferase -
I6H02_RS19080 3712111..3712851 - 741 WP_001225679.1 murein L,D-transpeptidase -
I6H02_RS19085 3713007..3713285 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
I6H02_RS19090 3713288..3713548 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
I6H02_RS19095 3713734..3714486 + 753 WP_143364582.1 C40 family peptidase -
I6H02_RS19100 3714543..3714899 - 357 WP_001030485.1 type II toxin-antitoxin system HicB family antitoxin -
I6H02_RS19105 3714892..3715170 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
I6H02_RS19110 3715275..3717368 - 2094 WP_001140828.1 flagellar biosynthesis protein FlhA -
I6H02_RS19115 3717352..3718491 - 1140 WP_000785662.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T182995 WP_000615976.1 NZ_CP065611:c3713285-3713007 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T182995 NZ_CP086625:c73734-73360 [Escherichia coli]
ATGAAAACATTACCCGATACACTCGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTGCTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT182995 WP_000729704.1 NZ_CP065611:c3713548-3713288 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT182995 NZ_CP086625:c74192-73824 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGATATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAACGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References