Detailed information of TA system TA182988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1613301..1614028
• Replicon: chromosome
• Accession: NZ_CP065611
• Organism: Escherichia coli strain FDAARGOS_945

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: I6H02_RS09035
• Protein ID: WP_000547564.1
• Coordinates: 1613717..1614028 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I6H02_RS09030
• Protein ID: WP_000126294.1
• Coordinates: 1613301..1613720 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H02_RS09010	1608350..1608877	-	528	WP_001078777.1	electron transport protein HydN	-
I6H02_RS09015	1609026..1610036	-	1011	WP_001343660.1	DNA-binding transcriptional regulator AscG	-
I6H02_RS09020	1610296..1611753	+	1458	WP_001107862.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
I6H02_RS09025	1611762..1613186	+	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
I6H02_RS09030	1613301..1613720	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
I6H02_RS09035	1613717..1614028	-	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
I6H02_RS09040	1614202..1614945	-	744	WP_001341829.1	hypothetical protein	-
I6H02_RS09045	1614988..1615458	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
I6H02_RS09050	1615451..1615861	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
I6H02_RS09055	1615858..1616625	-	768	WP_000067392.1	formate hydrogenlyase subunit HycG	-
I6H02_RS09060	1616625..1617167	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
I6H02_RS09065	1617177..1618886	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T182988 WP_000547564.1 NZ_CP065611:c1614028-1613717 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T182988 NZ_CP086624:3933122-3933340 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT182988 WP_000126294.1 NZ_CP065611:c1613720-1613301 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT182988 NZ_CP086624:3932722-3933096 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5