Detailed information of TA system TA182971

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 4735574..4735945
• Replicon: chromosome
• Accession: NZ_CP065607
• Organism: Escherichia coli strain FDAARGOS_946

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: I6H03_RS23180
• Protein ID: WP_001443846.1
• Coordinates: 4735796..4735945 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4735574..4735752 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H03_RS23150	4731326..4731496	+	171	WP_001625136.1	hypothetical protein	-
I6H03_RS23155	4731529..4732902	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
I6H03_RS23160	4733031..4733966	-	936	WP_000662472.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
I6H03_RS23165	4734018..4735253	-	1236	WP_000040839.1	site-specific integrase	-
I6H03_RS23170	4735255..4735470	-	216	WP_000079604.1	excisionase XisR	-
I6H03_RS23175	4735570..4735758	-	189	WP_001302840.1	DUF1187 family protein	-
-	4735574..4735752	+	179	NuclAT_0	-	Antitoxin
-	4735574..4735752	+	179	NuclAT_0	-	Antitoxin
-	4735574..4735752	+	179	NuclAT_0	-	Antitoxin
-	4735574..4735752	+	179	NuclAT_0	-	Antitoxin
I6H03_RS23180	4735796..4735945	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
I6H03_RS23185	4736001..4736810	-	810	WP_000166312.1	recombination protein RecT	-
I6H03_RS23190	4736803..4739475	-	2673	WP_000102127.1	exodeoxyribonuclease VIII	-
I6H03_RS23195	4739568..4739756	-	189	WP_000199475.1	DUF1482 family protein	-
I6H03_RS23200	4739753..4739941	-	189	WP_000449175.1	cell division inhibitor	-
I6H03_RS23205	4740431..4740583	-	153	WP_000379610.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4734018..4778277	44259

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T182971 WP_001443846.1 NZ_CP065607:c4735945-4735796 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T182971 NZ_CP086624:206651-206836 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT182971 NZ_CP065607:4735574-4735752 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin