Detailed information of TA system TA182968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3348390..3349084
• Replicon: chromosome
• Accession: NZ_CP065607
• Organism: Escherichia coli strain FDAARGOS_946

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: I6H03_RS16440
• Protein ID: WP_001263495.1
• Coordinates: 3348686..3349084 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: I6H03_RS16435
• Protein ID: WP_000554757.1
• Coordinates: 3348390..3348683 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H03_RS16415	3344013..3344510	+	498	Protein_3105	REP-associated tyrosine transposase RayT	-
I6H03_RS16420	3344743..3346455	-	1713	Protein_3106	flagellar biosynthesis protein FlhA	-
I6H03_RS16425	3346427..3347212	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
I6H03_RS16430	3347283..3348338	+	1056	WP_001226186.1	DNA polymerase IV	-
I6H03_RS16435	3348390..3348683	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
I6H03_RS16440	3348686..3349084	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
I6H03_RS16445	3349094..3349546	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
I6H03_RS16450	3349865..3350068	+	204	Protein_3112	RNA ligase RtcB family protein	-
I6H03_RS16455	3350067..3350588	+	522	Protein_3113	peptide chain release factor H	-
I6H03_RS16460	3350645..3352102	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
I6H03_RS16465	3352363..3352821	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	3353417..3353497	+	81	NuclAT_12	-	-
-	3353417..3353497	+	81	NuclAT_12	-	-
-	3353417..3353497	+	81	NuclAT_12	-	-
-	3353417..3353497	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T182968 WP_001263495.1 NZ_CP065607:3348686-3349084 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T182968 NZ_CP086623:c4749401-4749228 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT182968 WP_000554757.1 NZ_CP065607:3348390-3348683 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT182968 NZ_CP086623:c4749725-4749429 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: S1FJN6