Detailed information of TA system TA182967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 3342337..3342878
• Replicon: chromosome
• Accession: NZ_CP065607
• Organism: Escherichia coli strain FDAARGOS_946

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: S1FHQ9
• Locus tag: I6H03_RS16400
• Protein ID: WP_000615979.1
• Coordinates: 3342337..3342615 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: I6H03_RS16405
• Protein ID: WP_000729705.1
• Coordinates: 3342618..3342878 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H03_RS16385	3340020..3340598	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
I6H03_RS16390	3340703..3341470	+	768	WP_001301698.1	class II glutamine amidotransferase	-
I6H03_RS16395	3341441..3342181	-	741	WP_001225679.1	murein L,D-transpeptidase	-
I6H03_RS16400	3342337..3342615	-	279	WP_000615979.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
I6H03_RS16405	3342618..3342878	-	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
I6H03_RS16410	3343064..3343837	+	774	WP_000543891.1	C40 family peptidase	-
I6H03_RS16415	3344013..3344510	+	498	Protein_3105	REP-associated tyrosine transposase RayT	-
I6H03_RS16420	3344743..3346455	-	1713	Protein_3106	flagellar biosynthesis protein FlhA	-
I6H03_RS16425	3346427..3347212	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10830.62 Da
• Isoelectric Point: 10.0702

>T182967 WP_000615979.1 NZ_CP065607:c3342615-3342337 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T182967 NZ_CP086623:c4652441-4652091 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT182967 WP_000729705.1 NZ_CP065607:c3342878-3342618 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT182967 NZ_CP086623:c4652686-4652435 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: S1FHQ9