Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 171059..171594 Replicon plasmid unnamed1
Accession NZ_CP065525
Organism Enterococcus faecium strain VRE3389

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag ISU51_RS15360 Protein ID WP_002314366.1
Coordinates 171322..171594 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag ISU51_RS15355 Protein ID WP_002297422.1
Coordinates 171059..171322 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU51_RS15340 166486..168735 + 2250 WP_002314387.1 alpha-galactosidase -
ISU51_RS15345 168738..169913 + 1176 WP_002336205.1 MFS transporter -
ISU51_RS15350 169916..170825 - 910 Protein_182 IS982 family transposase -
ISU51_RS15355 171059..171322 + 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ISU51_RS15360 171322..171594 + 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
ISU51_RS15365 171671..172000 + 330 WP_002292678.1 AbrB family transcriptional regulator -
ISU51_RS15370 172055..172277 + 223 Protein_186 type II toxin-antitoxin system RelE/ParE family toxin -
ISU51_RS15375 172561..173418 + 858 WP_002292680.1 AAA family ATPase -
ISU51_RS15380 173419..173607 + 189 Protein_188 hypothetical protein -
ISU51_RS15385 173666..174346 - 681 WP_002331383.1 IS6-like element IS1216 family transposase -
ISU51_RS15390 174422..175065 - 644 Protein_190 transposase -
ISU51_RS15395 175340..175882 + 543 WP_002298088.1 hypothetical protein -
ISU51_RS15400 176038..176487 + 450 WP_010729386.1 TlpA family protein disulfide reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..227210 227210
- inside IScluster/Tn - - 160738..183156 22418


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T182873 WP_002314366.1 NZ_CP065525:171322-171594 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T182873 NZ_CP086618:c882486-882109 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGTACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT182873 WP_002297422.1 NZ_CP065525:171059-171322 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT182873 NZ_CP086618:c882944-882576 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References