Detailed information of TA system TA182868

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 473262..473776
• Replicon: chromosome
• Accession: NZ_CP065522
• Organism: Limosilactobacillus fermentum strain YLF016

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: I6U31_RS02350
• Protein ID: WP_069775721.1
• Coordinates: 473513..473776 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: C0WV67
• Locus tag: I6U31_RS02345
• Protein ID: WP_003684069.1
• Coordinates: 473262..473516 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6U31_RS02325 (I6U31_02325)	468903..469190	-	288	WP_003688751.1	transposase	-
I6U31_RS02330 (I6U31_02330)	469275..469706	-	432	Protein_447	helix-turn-helix domain-containing protein	-
I6U31_RS02335 (I6U31_02335)	469929..471299	+	1371	WP_070447884.1	FAD-dependent oxidoreductase	-
I6U31_RS02340 (I6U31_02340)	471681..473031	+	1351	Protein_449	transposase	-
I6U31_RS02345 (I6U31_02345)	473262..473516	+	255	WP_003684069.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
I6U31_RS02350 (I6U31_02350)	473513..473776	+	264	WP_069775721.1	Txe/YoeB family addiction module toxin	Toxin
I6U31_RS02355 (I6U31_02355)	474186..475307	-	1122	WP_023465987.1	PTS sugar transporter subunit IIC	-
I6U31_RS02360 (I6U31_02360)	475795..476514	-	720	WP_069775720.1	hypothetical protein	-
I6U31_RS02365 (I6U31_02365)	477375..478415	+	1041	WP_216285059.1	Gfo/Idh/MocA family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (26-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-55)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10303.00 Da
• Isoelectric Point: 10.3799

>T182868 WP_069775721.1 NZ_CP065522:473513-473776 [Limosilactobacillus fermentum]
MSYAIKLKRSANKELKKIKGSYLEKSFLQIIEQLRKDTLAPNQGFEKLVPPIKGFYSRRINIQHRLVYKVDQDTQTVIIY
SAWSHYE

Nucleotide

• Length: 264 bp

>T182868 NZ_CP086618:206651-206836 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9745.04 Da
• Isoelectric Point: 4.4237

>AT182868 WP_003684069.1 NZ_CP065522:473262-473516 [Limosilactobacillus fermentum]
MDQIVTPTQARKDLFKIIKRINENNEPVVIKPTKADEKSVVVIGEDDWKAIQETLFLVNHGVDKQIKERENEPLDDFDDV
WGQL

Nucleotide

• Length: 255 bp

>AT182868 NZ_CP086618:206853-207263 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGTGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A806T868