Detailed information of TA system TA182832

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 54147..54573
• Replicon: plasmid pPinheadLarry_05
• Accession: NZ_CP065472
• Organism: Klebsiella quasipneumoniae strain Pinhead Larry

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: I5M56_RS26380
• Protein ID: WP_001312861.1
• Coordinates: 54147..54305 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	54349..54573 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M56_RS26350	49197..49424	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
I5M56_RS26355	49518..50204	-	687	WP_000332484.1	PAS domain-containing protein	-
I5M56_RS26360	50395..50778	-	384	WP_000124981.1	relaxosome protein TraM	-
I5M56_RS26365	51055..51702	+	648	WP_031943493.1	transglycosylase SLT domain-containing protein	-
I5M56_RS26370	51999..52820	-	822	WP_001234469.1	DUF945 domain-containing protein	-
I5M56_RS26375	52938..53225	-	288	WP_000107544.1	hypothetical protein	-
I5M56_RS26380	54147..54305	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	54349..54573	-	225	NuclAT_0	-	Antitoxin
-	54349..54573	-	225	NuclAT_0	-	Antitoxin
-	54349..54573	-	225	NuclAT_0	-	Antitoxin
-	54349..54573	-	225	NuclAT_0	-	Antitoxin
I5M56_RS26385	54585..55304	-	720	WP_013362833.1	plasmid SOS inhibition protein A	-
I5M56_RS26390	55301..55735	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
I5M56_RS26395	55790..57748	-	1959	WP_001825193.1	ParB/RepB/Spo0J family partition protein	-
I5M56_RS26400	57814..58047	-	234	WP_000005990.1	DUF905 family protein	-
I5M56_RS26405	58110..58649	-	540	WP_001825195.1	single-stranded DNA-binding protein	-
I5M56_RS26410	58675..58881	-	207	WP_000275858.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / sul3	-	1..62418	62418

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T182832 WP_001312861.1 NZ_CP065472:c54305-54147 [Klebsiella quasipneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T182832 NZ_CP086610:1022281-1022679 [Shinella zoogloeoides]
ATGCTGGCCTATATGCTCGACACGAATATCTGCATCTATGTGATGAAGACCTATCCGCCTGCGGTTCGCGAGAAGTTCAA
CGCGCTGGCGGAGCAGCTTTGCATATCGAGCATCACGCTCGCGGAACTGCATTACGGCGCGGAAAAATCGGCGCGCCGGG
CTGAGAACCTCATGGCGATCGAGCACTTTGTCGCGCGGCTGGAGGTTCTGCCCTTCGCGGCCAGGGCCGCCGCGCATTAC
GGGCAGGTTCGGGCGGAGCTTGAAAGGGCCGGCACGCCATGCGGTCCGCACGACATGCAGATCGGCGGCCATGCCCGCAG
CGAAGGCTTGATCGTCGTGACGAACAATCTCAGGGAGTTTGCGCGCATGCCGGGGCTAAGGGCCGAGAACTGGCTGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT182832 NZ_CP065472:c54573-54349 [Klebsiella quasipneumoniae]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin