Detailed information of TA system TA182809

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 54613..54866
• Replicon: plasmid pOlaf_02
• Accession: NZ_CP065466
• Organism: Klebsiella pneumoniae strain Olaf

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: I5M57_RS28335
• Protein ID: WP_001312851.1
• Coordinates: 54613..54762 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	54807..54866 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M57_RS28300	49972..50387	-	416	Protein_64	IS1 family transposase	-
I5M57_RS28305	50636..51037	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
I5M57_RS28310	50970..51227	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
I5M57_RS28315	51320..51973	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
I5M57_RS28320	52912..53769	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
I5M57_RS28325	53762..53836	-	75	WP_001375168.1	RepA leader peptide Tap	-
I5M57_RS28330	54081..54329	-	249	WP_000083837.1	replication regulatory protein RepA	-
I5M57_RS28335	54613..54762	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	54807..54866	+	60	NuclAT_0	-	Antitoxin
-	54807..54866	+	60	NuclAT_0	-	Antitoxin
-	54807..54866	+	60	NuclAT_0	-	Antitoxin
-	54807..54866	+	60	NuclAT_0	-	Antitoxin
I5M57_RS28340	55067..55297	-	231	WP_001736714.1	hypothetical protein	-
I5M57_RS28345	55461..56060	-	600	WP_032083981.1	hypothetical protein	-
I5M57_RS28350	56446..56646	-	201	WP_015059022.1	hypothetical protein	-
I5M57_RS28355	56778..57338	-	561	WP_000139328.1	fertility inhibition protein FinO	-
I5M57_RS28360	57393..58139	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
I5M57_RS28365	58159..58320	-	162	Protein_77	DNA helicase	-
I5M57_RS28370	58384..59088	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
I5M57_RS28375	59141..59206	+	66	Protein_79	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2 / blaCTX-M-65 / tet(A)	-	1..105091	105091

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T182809 WP_001312851.1 NZ_CP065466:c54762-54613 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T182809 NZ_CP086604:583169-583272 [Escherichia fergusonii]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 60 bp

>AT182809 NZ_CP065466:54807-54866 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin