Detailed information of TA system TA182807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1773..2430
• Replicon: plasmid pOlaf_01
• Accession: NZ_CP065465
• Organism: Klebsiella pneumoniae strain Olaf

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: I5M57_RS27135
• Protein ID: WP_000270043.1
• Coordinates: 2080..2430 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I5M57_RS27130
• Protein ID: WP_000124640.1
• Coordinates: 1773..2075 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M57_RS27110	1..277	+	277	Protein_0	hypothetical protein	-
I5M57_RS27115	508..1035	+	528	WP_001236377.1	thermonuclease family protein	-
I5M57_RS27120	1093..1365	+	273	WP_001043047.1	HU family DNA-binding protein	-
I5M57_RS27125	1453..1746	+	294	WP_001239997.1	chromosome segregation protein ParM	-
I5M57_RS27130	1773..2075	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
I5M57_RS27135	2080..2430	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5M57_RS27140	2593..3141	+	549	WP_001061574.1	transcriptional regulator	-
I5M57_RS27145	3482..3676	+	195	WP_000343597.1	hypothetical protein	-
I5M57_RS27150	3687..4058	+	372	WP_000516916.1	hypothetical protein	-
I5M57_RS27155	4051..4521	+	471	WP_001281821.1	hypothetical protein	-
I5M57_RS27160	4536..4871	-	336	WP_000683476.1	hypothetical protein	-
I5M57_RS27165	4968..5456	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
I5M57_RS27170	5459..5956	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	rmtB / aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-14	-	1..139648	139648

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T182807 WP_000270043.1 NZ_CP065465:c2430-2080 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T182807 NZ_CP086594:c2662596-2662342 [Sphingomonas radiodurans]
ATGCAGTCGCTCAGCATCTCCGAAGCCCGCGCCAATATGAAAGCGCTCGTGGACCGCGTGGTCGCCGACAAAGCGCCGGT
GTCGATCACCCGTCAGAAGGGCGAGGGCGTGGTAATGATCTCGCAAAGCGAATGGTCGTCGATCGAGGAGACGCTCTATC
TCCTGCAATCGCCGGCGAACGCGAAGCGCCTTCTGGAAAGCATCGGCGAGCTTGATGCCGGGGCGGGCGTGGAGCGCGCG
CTGATCGAACCGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT182807 WP_000124640.1 NZ_CP065465:c2075-1773 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT182807 NZ_CP086594:c2662345-2662079 [Sphingomonas radiodurans]
GTGATCCTGACGTTTTCGTCGCGTGCCTGGTCCGAGTATCTTGAATGGCAGGGTGACGACCGGCGAGGTCTGGAGCGCGT
CAACGCGTTGATCAAGGAGTGCCAGCGCGATCCGTTCCGCGGCACCGGCAAGCCAGAGCCGCTTGGCGGTAATTTATCTG
GCTGGTGGTCCCGCCGCATCAATCGCGAGCATCGGCTGGTGTATCGGGTGAGCGGTGCCGGCGACGCGCAGGCGCTGGAG
ATCGCGCAGTGCCGGTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9