Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 4999393..4999979 Replicon chromosome
Accession NZ_CP065464
Organism Klebsiella pneumoniae strain Olaf

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag I5M57_RS24830 Protein ID WP_002920800.1
Coordinates 4999393..4999761 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag I5M57_RS24835 Protein ID WP_002920802.1
Coordinates 4999758..4999979 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M57_RS24810 4994896..4995966 - 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
I5M57_RS24815 4995968..4996813 - 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
I5M57_RS24820 4996810..4997697 - 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
I5M57_RS24825 4997804..4999120 - 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
I5M57_RS24830 4999393..4999761 - 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
I5M57_RS24835 4999758..4999979 - 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
I5M57_RS24840 5000143..5000856 - 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
I5M57_RS24845 5000858..5001625 - 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
I5M57_RS24850 5001622..5002899 - 1278 WP_002920806.1 branched chain amino acid ABC transporter permease LivM -
I5M57_RS24855 5002896..5003822 - 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4994159..5014747 20588


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-49)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T182805 WP_002920800.1 NZ_CP065464:c4999761-4999393 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T182805 NZ_CP086593:c44613-44344 [Enterococcus faecalis]
ATGTCGTATCGATTAGAATTTACTCCTGATGTAAAAAAACAGTTAAAAAAAATGGATAAATATCAAGCGACATTGCTAAC
AAGATGGTTGTATCGAAATATTGATGGAACGGCCAATCCTAGAGAGCATGGAAAAGCTTTATCAGCAAATCGTGCAGGTC
AGTGGCGATATAGGATAGGGAATTATCGTGTCATTGTAGAAATAGAAGATGAACGTCTTGTTGTTACAGCCATTCAGGTT
GGCCACAGAAGAAATGTTTACGACAAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT182805 WP_002920802.1 NZ_CP065464:c4999979-4999758 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT182805 NZ_CP086593:c44840-44613 [Enterococcus faecalis]
ATGGCTATGATTACTGTAAGAGTGAGTGATGAAGAAAAAGAATGGCTTTCTTACATGGCAGAATTTTTCGGTGTGACGTT
GTCGGATTTGTTAAAAAGCTATTCAATGGAACAATTAGAAGATGAGTACGATAAACAAACAGCAGAAATTGCTCACAAAC
GATGGATTGAGCAAGGAAAAGAAACAATTTCTATGGACGAAATATTAAATGAGTTTGGTGGATTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References