Detailed information of TA system TA182788

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 24691..25348
• Replicon: plasmid pGordon_03
• Accession: NZ_CP065463
• Organism: Klebsiella pneumoniae strain Gordon

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: I5M58_RS29330
• Protein ID: WP_000270043.1
• Coordinates: 24691..25041 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I5M58_RS29335
• Protein ID: WP_000124640.1
• Coordinates: 25046..25348 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M58_RS29295	21165..21662	-	498	WP_000062185.1	hypothetical protein	-
I5M58_RS29300	21665..22153	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
I5M58_RS29305	22250..22585	+	336	WP_000683476.1	hypothetical protein	-
I5M58_RS29310	22600..23070	-	471	WP_001281821.1	hypothetical protein	-
I5M58_RS29315	23063..23434	-	372	WP_000516916.1	hypothetical protein	-
I5M58_RS29320	23445..23639	-	195	WP_000343597.1	hypothetical protein	-
I5M58_RS29325	23980..24528	-	549	WP_001061574.1	transcriptional regulator	-
I5M58_RS29330	24691..25041	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5M58_RS29335	25046..25348	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
I5M58_RS29340	25375..25668	-	294	WP_001239997.1	chromosome segregation protein ParM	-
I5M58_RS29345	25756..26028	-	273	WP_001043047.1	HU family DNA-binding protein	-
I5M58_RS29350	26086..26613	-	528	WP_001236377.1	thermonuclease family protein	-
I5M58_RS29355	26844..27701	-	858	WP_001167032.1	hypothetical protein	-
I5M58_RS29360	27688..27918	-	231	WP_000972663.1	hypothetical protein	-
I5M58_RS29365	27918..28436	-	519	WP_000210756.1	nitrite reductase	-
I5M58_RS29370	28433..28879	-	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
I5M58_RS29375	28879..29238	-	360	WP_000422768.1	hypothetical protein	-
I5M58_RS29380	29295..29723	-	429	WP_000591074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / rmtB / blaCTX-M-14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B	-	1..139635	139635

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T182788 WP_000270043.1 NZ_CP065463:24691-25041 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T182788 NZ_CP086574:c855978-855790 [Staphylococcus argenteus]
ATGACTTTACATTTTGCAATTTTATTTTGGCTAGCATTAATTTTTATCGTTGCTGGTACATTTATATTTGTCTTAATGAA
AAAAACTGCTAAAGAATCTAAAAAAGAATCTTATTTAAGCTTCGCTGTTATTTTCTATATTTTTGGATTCGCAATGCTAA
TTTATACATTAATATTTGGAATTCTTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT182788 WP_000124640.1 NZ_CP065463:25046-25348 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT182788 NZ_CP086574:c856267-856007 [Staphylococcus argenteus]
ATGAAAAACATTCAATTAAATGCTATAAATTTAGTTATAACAATCATATTTGCTATCTTTAACATTATGATTACATATAA
CAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGCATTATCATTTGTGGTTCGATACTCATTGTATCGTTTACCATTG
CAATGATTACTAAATTCTGGTTAAGTGAAATATTATTTTTTATTAACATCGTGCTCGTTCTTTATTATATTTATCCAATT
TTTTATGATTTCATTAATTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9