Detailed information of TA system TA182783

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 49396..49649
• Replicon: plasmid pGordon_01
• Accession: NZ_CP065461
• Organism: Klebsiella pneumoniae strain Gordon

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: I5M58_RS27740
• Protein ID: WP_001312851.1
• Coordinates: 49396..49545 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	49590..49649 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M58_RS27705	44755..45170	-	416	Protein_57	IS1 family transposase	-
I5M58_RS27710	45419..45820	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
I5M58_RS27715	45753..46010	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
I5M58_RS27720	46103..46756	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
I5M58_RS27725	47695..48552	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
I5M58_RS27730	48545..48619	-	75	WP_001375168.1	RepA leader peptide Tap	-
I5M58_RS27735	48864..49112	-	249	WP_000083837.1	replication regulatory protein RepA	-
I5M58_RS27740	49396..49545	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	49590..49649	+	60	NuclAT_0	-	Antitoxin
-	49590..49649	+	60	NuclAT_0	-	Antitoxin
-	49590..49649	+	60	NuclAT_0	-	Antitoxin
-	49590..49649	+	60	NuclAT_0	-	Antitoxin
I5M58_RS27745	49850..50080	-	231	WP_001736714.1	hypothetical protein	-
I5M58_RS27750	50244..50843	-	600	WP_032083981.1	hypothetical protein	-
I5M58_RS27755	51229..51429	-	201	WP_015059022.1	hypothetical protein	-
I5M58_RS27760	51561..52121	-	561	WP_000139328.1	fertility inhibition protein FinO	-
I5M58_RS27765	52176..52922	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
I5M58_RS27770	52942..53103	-	162	Protein_70	DNA helicase	-
I5M58_RS27775	53167..53871	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
I5M58_RS27780	53924..53989	+	66	Protein_72	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2 / blaCTX-M-65 / tet(A)	-	1..105090	105090

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T182783 WP_001312851.1 NZ_CP065461:c49545-49396 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T182783 NZ_CP086572:2371438-2371545 [Staphylococcus argenteus]
ATGTTCAATTTATTGATTAACATCATGACTACAGCTATAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT182783 NZ_CP065461:49590-49649 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin