Detailed information of TA system TA182741

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 157397..158054
• Replicon: plasmid pBR_01
• Accession: NZ_CP065454
• Organism: Klebsiella pneumoniae strain Beach Ranger

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: I5M60_RS27430
• Protein ID: WP_000270043.1
• Coordinates: 157704..158054 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I5M60_RS27425
• Protein ID: WP_000124640.1
• Coordinates: 157397..157699 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M60_RS27380	153022..153450	+	429	WP_000591074.1	hypothetical protein	-
I5M60_RS27385	153507..153866	+	360	WP_000422768.1	hypothetical protein	-
I5M60_RS27390	153866..154312	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
I5M60_RS27395	154309..154827	+	519	WP_000210756.1	nitrite reductase	-
I5M60_RS27400	154827..155057	+	231	WP_000972663.1	hypothetical protein	-
I5M60_RS27405	155044..155901	+	858	WP_001167032.1	hypothetical protein	-
I5M60_RS27410	156132..156659	+	528	WP_001236377.1	thermonuclease family protein	-
I5M60_RS27415	156717..156989	+	273	WP_001043047.1	HU family DNA-binding protein	-
I5M60_RS27420	157077..157370	+	294	WP_001239997.1	chromosome segregation protein ParM	-
I5M60_RS27425	157397..157699	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
I5M60_RS27430	157704..158054	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5M60_RS27435	158217..158765	+	549	WP_001061574.1	transcriptional regulator	-
I5M60_RS27440	159106..159300	+	195	WP_000343597.1	hypothetical protein	-
I5M60_RS27445	159311..159682	+	372	WP_000516916.1	hypothetical protein	-
I5M60_RS27450	159675..160145	+	471	WP_001281821.1	hypothetical protein	-
I5M60_RS27455	160160..160495	-	336	WP_000683476.1	hypothetical protein	-
I5M60_RS27460	160592..161080	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
I5M60_RS27465	161083..161580	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / rmtB / tet(G) / floR / qacE / aadA2 / dfrA12 / aac(3)-IId / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-14 / erm(42)	-	1..176326	176326

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T182741 WP_000270043.1 NZ_CP065454:c158054-157704 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T182741 NZ_CP086558:c2827931-2827830 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT182741 WP_000124640.1 NZ_CP065454:c157699-157397 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT182741 NZ_CP086558:2827706-2827885 [Enterococcus faecalis]
TGAAAAGAGAGATATGCTACTACATACCTCTCCTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGT
TTTTGTTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACC
GAAAATCAGTAGTGCAACAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9