Detailed information of TA system TA182722

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 107929..108586
• Replicon: plasmid pOceanRanger_02
• Accession: NZ_CP065451
• Organism: Klebsiella pneumoniae strain Ocean Ranger

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: I5M61_RS29590
• Protein ID: WP_000270043.1
• Coordinates: 108236..108586 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I5M61_RS29585
• Protein ID: WP_000124640.1
• Coordinates: 107929..108231 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M61_RS29540	103554..103982	+	429	WP_000591074.1	hypothetical protein	-
I5M61_RS29545	104039..104398	+	360	WP_000422768.1	hypothetical protein	-
I5M61_RS29550	104398..104844	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
I5M61_RS29555	104841..105359	+	519	WP_000210756.1	nitrite reductase	-
I5M61_RS29560	105359..105589	+	231	WP_000972663.1	hypothetical protein	-
I5M61_RS29565	105576..106433	+	858	WP_001167032.1	hypothetical protein	-
I5M61_RS29570	106664..107191	+	528	WP_001236377.1	thermonuclease family protein	-
I5M61_RS29575	107249..107521	+	273	WP_001043047.1	HU family DNA-binding protein	-
I5M61_RS29580	107609..107902	+	294	WP_001239997.1	chromosome segregation protein ParM	-
I5M61_RS29585	107929..108231	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
I5M61_RS29590	108236..108586	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5M61_RS29595	108749..109297	+	549	WP_001061574.1	transcriptional regulator	-
I5M61_RS29600	109638..109832	+	195	WP_000343597.1	hypothetical protein	-
I5M61_RS29605	109843..110214	+	372	WP_000516916.1	hypothetical protein	-
I5M61_RS29610	110207..110677	+	471	WP_001281821.1	hypothetical protein	-
I5M61_RS29615	110692..111027	-	336	WP_000683476.1	hypothetical protein	-
I5M61_RS29620	111124..111612	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
I5M61_RS29625	111615..112112	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-14 / erm(42) / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	-	1..154765	154765

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T182722 WP_000270043.1 NZ_CP065451:c108586-108236 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T182722 NZ_CP086556:1855715-1855969 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT182722 WP_000124640.1 NZ_CP065451:c108231-107929 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT182722 NZ_CP086556:1855467-1855718 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9