Detailed information of TA system TA182697

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 61133..61386
• Replicon: plasmid pSeaSuperman_01
• Accession: NZ_CP065445
• Organism: Klebsiella pneumoniae strain Sea Superman

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: I5M62_RS27525
• Protein ID: WP_001312851.1
• Coordinates: 61237..61386 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	61133..61192 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M62_RS27485	56793..56858	-	66	Protein_75	helix-turn-helix domain-containing protein	-
I5M62_RS27490	56911..57615	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
I5M62_RS27495	57679..57840	+	162	Protein_77	DNA helicase	-
I5M62_RS27500	57860..58606	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
I5M62_RS27505	58661..59221	+	561	WP_000139328.1	fertility inhibition protein FinO	-
I5M62_RS27510	59353..59553	+	201	WP_015059022.1	hypothetical protein	-
I5M62_RS27515	59939..60538	+	600	WP_032083981.1	hypothetical protein	-
I5M62_RS27520	60702..60932	+	231	WP_001736714.1	hypothetical protein	-
-	61133..61192	-	60	NuclAT_0	-	Antitoxin
-	61133..61192	-	60	NuclAT_0	-	Antitoxin
-	61133..61192	-	60	NuclAT_0	-	Antitoxin
-	61133..61192	-	60	NuclAT_0	-	Antitoxin
I5M62_RS27525	61237..61386	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
I5M62_RS27530	61670..61918	+	249	WP_000083837.1	replication regulatory protein RepA	-
I5M62_RS27535	62163..62237	+	75	WP_001375168.1	RepA leader peptide Tap	-
I5M62_RS27540	62230..63087	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
I5M62_RS27545	64026..64679	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
I5M62_RS27550	64772..65029	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
I5M62_RS27555	64962..65363	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
I5M62_RS27560	65612..66027	+	416	Protein_90	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2	-	1..94245	94245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T182697 WP_001312851.1 NZ_CP065445:61237-61386 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T182697 NZ_CP086544:316247-316477 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT182697 NZ_CP065445:c61192-61133 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin