Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4836572..4837218 Replicon chromosome
Accession NZ_CP065444
Organism Klebsiella pneumoniae strain Sea Superman

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag I5M62_RS24080 Protein ID WP_002920560.1
Coordinates 4836871..4837218 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag I5M62_RS24075 Protein ID WP_002920557.1
Coordinates 4836572..4836871 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M62_RS24055 4832778..4833536 - 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -
I5M62_RS24060 4833586..4834416 - 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
I5M62_RS24065 4834467..4834796 - 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
I5M62_RS24070 4835001..4836509 + 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
I5M62_RS24075 4836572..4836871 - 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
I5M62_RS24080 4836871..4837218 - 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I5M62_RS24085 4837394..4839841 - 2448 WP_002920561.1 glycogen phosphorylase -
I5M62_RS24090 4839859..4841292 - 1434 WP_002920564.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T182694 WP_002920560.1 NZ_CP065444:c4837218-4836871 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T182694 NZ_CP086538:c4178119-4177946 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT182694 WP_002920557.1 NZ_CP065444:c4836871-4836572 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT182694 NZ_CP086538:c4178443-4178147 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References