Detailed information of TA system TA182678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 117703..118360
• Replicon: plasmid pPatrickStar_02
• Accession: NZ_CP065443
• Organism: Klebsiella pneumoniae strain Patrick Star

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: I5M63_RS27700
• Protein ID: WP_000270043.1
• Coordinates: 118010..118360 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: I5M63_RS27695
• Protein ID: WP_000124640.1
• Coordinates: 117703..118005 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M63_RS27650	113328..113756	+	429	WP_000591074.1	hypothetical protein	-
I5M63_RS27655	113813..114172	+	360	WP_000422768.1	hypothetical protein	-
I5M63_RS27660	114172..114618	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
I5M63_RS27665	114615..115133	+	519	WP_000210756.1	nitrite reductase	-
I5M63_RS27670	115133..115363	+	231	WP_000972663.1	hypothetical protein	-
I5M63_RS27675	115350..116207	+	858	WP_001167032.1	hypothetical protein	-
I5M63_RS27680	116438..116965	+	528	WP_001236377.1	thermonuclease family protein	-
I5M63_RS27685	117023..117295	+	273	WP_001043047.1	HU family DNA-binding protein	-
I5M63_RS27690	117383..117676	+	294	WP_001239997.1	chromosome segregation protein ParM	-
I5M63_RS27695	117703..118005	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
I5M63_RS27700	118010..118360	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I5M63_RS27705	118523..119071	+	549	WP_001061574.1	transcriptional regulator	-
I5M63_RS27710	119412..119606	+	195	WP_000343597.1	hypothetical protein	-
I5M63_RS27715	119617..119988	+	372	WP_000516916.1	hypothetical protein	-
I5M63_RS27720	119981..120451	+	471	WP_001281821.1	hypothetical protein	-
I5M63_RS27725	120466..120801	-	336	WP_000683476.1	hypothetical protein	-
I5M63_RS27730	120898..121386	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
I5M63_RS27735	121389..121886	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-14 / erm(42)	-	1..139394	139394

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T182678 WP_000270043.1 NZ_CP065443:c118360-118010 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T182678 NZ_CP086534:c42272-42120 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT182678 WP_000124640.1 NZ_CP065443:c118005-117703 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT182678 NZ_CP086534:42324-42384 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9