Detailed information of TA system TA182656

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 34683..34936
• Replicon: plasmid pMrsPuff_02
• Accession: NZ_CP065440
• Organism: Klebsiella pneumoniae strain Mrs. Puff

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: I5M55_RS28040
• Protein ID: WP_001312851.1
• Coordinates: 34683..34832 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	34877..34936 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M55_RS28005	30042..30457	-	416	Protein_41	IS1 family transposase	-
I5M55_RS28010	30706..31107	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
I5M55_RS28015	31040..31297	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
I5M55_RS28020	31390..32043	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
I5M55_RS28025	32982..33839	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
I5M55_RS28030	33832..33906	-	75	WP_001375168.1	RepA leader peptide Tap	-
I5M55_RS28035	34151..34399	-	249	WP_000083837.1	replication regulatory protein RepA	-
I5M55_RS28040	34683..34832	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	34877..34936	+	60	NuclAT_0	-	Antitoxin
-	34877..34936	+	60	NuclAT_0	-	Antitoxin
-	34877..34936	+	60	NuclAT_0	-	Antitoxin
-	34877..34936	+	60	NuclAT_0	-	Antitoxin
I5M55_RS28045	35137..35367	-	231	WP_001736714.1	hypothetical protein	-
I5M55_RS28050	35531..36130	-	600	WP_032083981.1	hypothetical protein	-
I5M55_RS28055	36516..36716	-	201	WP_015059022.1	hypothetical protein	-
I5M55_RS28060	36848..37408	-	561	WP_000139328.1	fertility inhibition protein FinO	-
I5M55_RS28065	37463..38209	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
I5M55_RS28070	38229..38390	-	162	Protein_54	DNA helicase	-
I5M55_RS28075	38454..39158	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
I5M55_RS28080	39211..39276	+	66	Protein_56	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaKPC-2 / blaCTX-M-90	-	1..89034	89034

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T182656 WP_001312851.1 NZ_CP065440:c34832-34683 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T182656 NZ_CP086526:2648986-2649093 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT182656 NZ_CP065440:34877-34936 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin